Asghari Ali, Mohammadi Mohammad Reza, Motazedian Mohammad Hossein, Asgari Qasem, Shamsi Laya, Eghball Mohsen
Social Determinants of Health Research Center, Research Institute for Prevention of Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran.
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Parasit Dis. 2025 Mar;49(1):242-247. doi: 10.1007/s12639-024-01727-6. Epub 2024 Aug 27.
Domestic animals play a vital role in the spread and transmission of various zoonotic agents to humans. , an important enteric zoonotic parasite, is widespread globally. This research aimed to assess the presence and genetic diversity of assemblages in domestic animals in Shiraz, southern Iran. A total of 245 fresh fecal samples were collected from 87 dogs, 63 cats, and 95 cattle in Shiraz, the capital city of Fars province, between July 2021 and August 2022. None of the animals showed gastrointestinal signs such as diarrhea, and all fecal samples had normal consistency. Upon parasite observation using light microscopy, a DNA fragment of gene was amplified using nested-PCR. Using direct wet mount and Wheatley's trichrome staining examination, 9 out of 245 samples (3.7%; 1 from dogs and 8 from cattle) tested positive for . Molecular methods confirmed 1.1% (1/87) of dogs and 8.4% (8/95) of cattle to be positive. Giardiasis was not detected in cats. Phylogenetic analysis revealed that isolates infecting dogs and cattle in Shiraz belonged to three genotypes/assemblages: A, B (zoonotic), and E (non-zoonotic). In cattle, assemblage E (75%; 6/8), A (12.5%; 1/8), and B (12.5%; 1/8) were identified, while dogs were infected with assemblage A (100%; 1/1). In Shiraz, southwestern Iran, cattle and dogs could potentially transmit infections to humans.
家畜在各种人畜共患病原体向人类的传播中起着至关重要的作用。贾第虫是一种重要的肠道人畜共患寄生虫，在全球广泛分布。本研究旨在评估伊朗南部设拉子家畜中贾第虫组合的存在情况和遗传多样性。2021年7月至2022年8月期间，从法尔斯省省会设拉子的87只狗、63只猫和95头牛中总共采集了245份新鲜粪便样本。没有动物表现出腹泻等胃肠道症状，所有粪便样本的质地均正常。使用光学显微镜观察寄生虫后，通过巢式PCR扩增贾第虫基因的DNA片段。通过直接湿片法和惠特利三色染色检查，245份样本中有9份（3.7%；1份来自狗，8份来自牛）贾第虫检测呈阳性。分子方法证实1.1%（1/87）的狗和8.4%（8/95）的牛呈阳性。在猫中未检测到贾第虫病。系统发育分析表明，设拉子感染狗和牛的贾第虫分离株属于三种基因型/组合：A、B（人畜共患）和E（非人畜共患）。在牛中，鉴定出组合E（75%；6/8）、A（12.5%；1/8）和B（12.5%；1/8），而狗感染的是组合A（100%；1/1）。在伊朗西南部的设拉子，牛和狗可能会将贾第虫感染传播给人类。
