Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Int J Biol Macromol. 2022 Jul 1;212:527-535. doi: 10.1016/j.ijbiomac.2022.05.100. Epub 2022 May 14.
Using electrospun chitosan/polyethylene oxide nanofibers mat (CS/PEO NFM) as carrier, Cu@CS/PEO NFM, a new tetracycline (TC) solid-state colorimetric sensor, were simply prepared by directly immobilizing Cu on surface of CS/PEO NFM. After immersing in TC solutions, Cu@CS/PEO NFM can display visible color changes to the naked eye within 5.0 min, and the TC concentration-dependent color changes can also be quantitatively analyzed with a smartphone. Because Cu can enhance the adsorption of CS/PEO NFM for TC, Cu@CS/PEO NFM possess a more sensitive response ability to TC, ensuring that the colorimetric sensing detection will not be interfered by other coexisting drugs. Due to the reversible combination of chitosan and Cu, the colorimetric sensing ability of Cu@CS/PEO NFM can be regenerated even after being reused at least 4 times. In addition, the naked eye detection limit of Cu@CS/PEO NFM-based colorimetric sensing is 65 μg kg, which does not exceed the maximum residue limit in milk samples set by China (100 μg kg). The obtained results fully indicated the practical application value of this method in preventing the hazard of TC residues.
采用静电纺丝壳聚糖/聚氧化乙烯纳米纤维垫（CS/PEO NFM）作为载体，通过直接将 Cu 固定在 CS/PEO NFM 表面，简单制备了载 Cu 的 CS/PEO NFM（Cu@CS/PEO NFM），这是一种新型的四环素（TC）固态比色传感器。将 Cu@CS/PEO NFM 浸入 TC 溶液中后，肉眼可在 5.0 分钟内观察到明显的颜色变化，同时智能手机可定量分析 TC 浓度依赖的颜色变化。由于 Cu 可以增强 CS/PEO NFM 对 TC 的吸附，因此 Cu@CS/PEO NFM 对 TC 具有更灵敏的响应能力，从而确保比色传感检测不会受到其他共存药物的干扰。由于壳聚糖和 Cu 之间的可逆结合，Cu@CS/PEO NFM 的比色传感能力即使在重复使用至少 4 次后也可以再生。此外，基于 Cu@CS/PEO NFM 的比色传感的肉眼检测限为 65μgkg，未超过中国（100μgkg）设定的牛奶中 TC 残留量的最大限量。所得结果充分表明了该方法在防止 TC 残留危害方面的实际应用价值。
