College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China; College of Life Science, Jilin Agricultural University, Changchun, China.
College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun 130118, China; Department of Animal Production, Faculty of Agriculture, Al-Azhar University, Cairo, 11884, Egypt.
Fish Shellfish Immunol. 2022 Jun;125:258-265. doi: 10.1016/j.fsi.2022.05.008. Epub 2022 May 14.
The purpose of this study was to investigate the effect of Bacillus amyloliquefaciens LSG2-8 on the growth performance, immune function, antioxidant capacity, and disease resistance of Rhynchocypris lagowskii. Fish were fed with the feed containing five levels such as 0, 1.0 × 10, 1.0 × 10, 1.0 × 10 and 1.0 × 10 CFU/g of the B. amyloliquefaciens LSG2-8 for 56 days. After 56 days of feeding, twenty four fish were randomly selected to test various growth, immune and antioxidant parameters. Ten fish were challenged with Aeromonas hydrophila for 14 days; the mortality rate was recorded 14 days after infection. The results showed that B. amyloliquefaciens LSG2-8 could significantly increase the growth parameters of R. lagowskii's, such as final body weight, weight gain rate, specific growth rate, and feed efficiency (p < 0.05). Further examination revealed the activity of antioxidant enzymes, Nrf-2 mRNA, and Keap-1 mRNA gene expression in the intestine and liver, and the serum immune index of R. lagowskii in the 1.0 × 108 CFU/g were all significantly higher compared to the other groups. Furthermore, fish fed a diet supplemented with B. amyloliquefaciens LSG2-8 had a significantly lower (p < 0.05) post-challenge mortality rate than the control fish. In summary, the research results showed that B. amyloliquefaciens LSG2-8 could improve the growth performance, immune function, antioxidant capacity, and disease resistance of R. lagowskii and be used in aquaculture.
本研究旨在探讨解淀粉芽孢杆菌 LSG2-8 对瓦氏黄颡鱼生长性能、免疫功能、抗氧化能力和抗病力的影响。将鱼分别投喂含 0、1.0×10、1.0×10、1.0×10 和 1.0×10 CFU/g 解淀粉芽孢杆菌 LSG2-8 的饲料 56 天。56 天后，随机选取 24 尾鱼进行生长、免疫和抗氧化参数的测定。选取 10 尾鱼进行嗜水气单胞菌攻毒实验，感染后第 14 天记录死亡率。结果表明，解淀粉芽孢杆菌 LSG2-8 能显著提高瓦氏黄颡鱼的生长性能，如末重、增重率、特定生长率和饲料效率（p<0.05）。进一步研究发现，1.0×108 CFU/g 组瓦氏黄颡鱼肠道和肝脏的抗氧化酶活性、Nrf-2 mRNA 和 Keap-1 mRNA 基因表达及血清免疫指标均显著高于其他组。此外，摄食添加解淀粉芽孢杆菌 LSG2-8 饲料的鱼的攻毒后死亡率显著低于对照组（p<0.05）。综上所述，研究结果表明，解淀粉芽孢杆菌 LSG2-8 能提高瓦氏黄颡鱼的生长性能、免疫功能、抗氧化能力和抗病力，可用于水产养殖。
