Effects of on growth, intestinal flora and metabolism of .

The incorporation of probiotics into the diet of large yellow croaker has been demonstrated by several studies to confer partial disease resistance. isolated from the intestinal flora was used to study its effects on performance growth indicators, intestinal tissue structure, intestinal flora and the metabolism of . A total of 180 fishes with an initial body weight of (164.00 ± 54.00) g were fed diets with three different concentrations of : 0 cfu/mL (FC0, control group), 10 cfu/mL (FC8, treatment group), and 10 cfu/mL (FC12, treatment group). The results showed that there were no significant differences in specific growth rate among all groups ( > 0.05). Compared to the FC0 group, the final body weight and Weight gain rate were significantly higher in FC8 and FC12 groups ( < 0.05). The Survival of the FC12 group significantly improved ( < 0.05). Compared to the FC0 group, crude protein content in muscle of the FC8 group significantly increased ( < 0.05), crude fat content significantly increased in the FC12 group ( < 0.05), crude protein content in whole fish experimental groups significantly increased ( < 0.05), and ash content significantly increased in the FC8 group ( < 0.05). In terms of antioxidant ability, the content of LZM in blood increased significantly in the FC8 group ( < 0.05), GSH content in liver of the FC12 group increased significantly ( < 0.05), while the content of MDA and AKP in blood and liver had no significant difference ( > 0.05). At the level of intestinal structure, there were no significant differences in villus height, crypt depth and goblet cell number between control group and treatment groups ( > 0.05). At the phylum level, Firmicutes was the dominant phylum, and the genus level, and were the dominant bacteria in FC8 and FC12. A total of 1070 metabolites were identified, among which lipid metabolites accounted for 46.7%. Metabolites were involved in six main ways, mainly related to the metabolism of amino acids and lipids. The correlation analysis between microbes and metabolites showed that the intestinal flora of could promote the synthesis of metabolites, among which and could promote the synthesis of beneficial metabolites such as amino acids and vitamins. Through this study, we found that can significantly improve growth, the antioxidant immunity ability and promote the expression of growth related metabolites, with the FC12 group being the better successful.