Huang Ling, Shui Xiaomei, Wang Hanying, Qiu Haoyu, Tao Chenzhi, Yin Heng, Wang Ping
College of Marine Science and Technology, Zhejiang Ocean University, 316022, Zhoushan, China.
National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, 316022, Zhoushan, China.
Biochem Biophys Rep. 2023 Sep 12;35:101546. doi: 10.1016/j.bbrep.2023.101546. eCollection 2023 Sep.
The incorporation of probiotics into the diet of large yellow croaker has been demonstrated by several studies to confer partial disease resistance. isolated from the intestinal flora was used to study its effects on performance growth indicators, intestinal tissue structure, intestinal flora and the metabolism of . A total of 180 fishes with an initial body weight of (164.00 ± 54.00) g were fed diets with three different concentrations of : 0 cfu/mL (FC0, control group), 10 cfu/mL (FC8, treatment group), and 10 cfu/mL (FC12, treatment group). The results showed that there were no significant differences in specific growth rate among all groups ( > 0.05). Compared to the FC0 group, the final body weight and Weight gain rate were significantly higher in FC8 and FC12 groups ( < 0.05). The Survival of the FC12 group significantly improved ( < 0.05). Compared to the FC0 group, crude protein content in muscle of the FC8 group significantly increased ( < 0.05), crude fat content significantly increased in the FC12 group ( < 0.05), crude protein content in whole fish experimental groups significantly increased ( < 0.05), and ash content significantly increased in the FC8 group ( < 0.05). In terms of antioxidant ability, the content of LZM in blood increased significantly in the FC8 group ( < 0.05), GSH content in liver of the FC12 group increased significantly ( < 0.05), while the content of MDA and AKP in blood and liver had no significant difference ( > 0.05). At the level of intestinal structure, there were no significant differences in villus height, crypt depth and goblet cell number between control group and treatment groups ( > 0.05). At the phylum level, Firmicutes was the dominant phylum, and the genus level, and were the dominant bacteria in FC8 and FC12. A total of 1070 metabolites were identified, among which lipid metabolites accounted for 46.7%. Metabolites were involved in six main ways, mainly related to the metabolism of amino acids and lipids. The correlation analysis between microbes and metabolites showed that the intestinal flora of could promote the synthesis of metabolites, among which and could promote the synthesis of beneficial metabolites such as amino acids and vitamins. Through this study, we found that can significantly improve growth, the antioxidant immunity ability and promote the expression of growth related metabolites, with the FC12 group being the better successful.
多项研究表明，将益生菌添加到大黄鱼的饮食中可赋予其部分抗病能力。从肠道菌群中分离出的[具体益生菌名称未给出]用于研究其对生长性能指标、肠道组织结构、肠道菌群以及[具体物质未给出]代谢的影响。选取180尾初始体重为(164.00 ± 54.00) g的大黄鱼，分别投喂含有三种不同浓度[具体益生菌名称未给出]的饲料：0 cfu/mL（FC0，对照组）、10⁸ cfu/mL（FC8，处理组）和10¹² cfu/mL（FC12，处理组）。结果表明，所有组之间的特定生长率无显著差异（P > 0.05）。与FC0组相比，FC8组和FC12组的终末体重和增重率显著更高（P < 0.05）。FC12组的存活率显著提高（P < 0.05）。与FC0组相比，FC8组肌肉中的粗蛋白含量显著增加（P < 0.05），FC12组的粗脂肪含量显著增加（P < 0.05），全鱼实验组的粗蛋白含量显著增加（P < 0.05），FC8组的灰分含量显著增加（P < 0.05）。在抗氧化能力方面，FC8组血液中LZM的含量显著增加（P < 0.05），FC12组肝脏中GSH的含量显著增加（P < 0.05），而血液和肝脏中MDA和AKP的含量无显著差异（P > 0.05）。在肠道结构水平上，对照组和处理组之间的绒毛高度、隐窝深度和杯状细胞数量无显著差异（P > 0.05）。在门水平上，厚壁菌门是优势菌门，在属水平上，[具体属名称未给出]是FC8组和FC12组中的优势细菌。共鉴定出1070种代谢产物，其中脂质代谢产物占46.7%。代谢产物主要通过六种主要方式参与，主要与氨基酸和脂质的代谢有关。微生物与代谢产物之间的相关性分析表明，[具体益生菌名称未给出]的肠道菌群可促进代谢产物的合成，其中[具体微生物名称未给出]可促进氨基酸和维生素等有益代谢产物的合成。通过本研究发现，[具体益生菌名称未给出]可显著提高生长性能、抗氧化免疫能力并促进生长相关代谢产物的表达，FC12组效果更佳。
