College of Animal Science and Technology, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, Jilin Agricultural University, Changchun, China.
Epros Kechuang (Liaoning) Co., Ltd, Shenyang, China.
J Fish Biol. 2023 Aug;103(2):220-234. doi: 10.1111/jfb.15382. Epub 2023 May 16.
Probiotics are widely used in aquaculture. This article aims to study the effect of Bacillus amyloliquefaciens LSG2-8 on the intestinal barrier function of Rhynchocypris lagowskii. B. amyloliquefaciens LSG2-8 were added to R. lagowskii basal diets (CK) as additives at four concentrations: 1.0 × 10 (D-6), 1.0 × 10 (D-7), 1.0 × 10 (D-8) and 1.0 × 10 (D-9) CFU g by dry weight of basal diet. After a 56-day feeding experiment, the activities of intestinal digestive enzymes and immunity-related enzymes of R. lagowskii on group D-6, D-7, D-8 and D-9 diet were significantly higher than the control (P < 0.05). In molecular experiments, the authors found that the levels of TGF-β mRNA, IL-10 mRNA, ZO-1 mRNA and claudin-3 mRNA in group D-8 R. lagowskii were significantly higher (P < 0.05) than those of the control and other groups. Furthermore, the levels of IL-1β and IL-8 mRNA of R. lagowskii on group D-6, D-7, D-8 and D-9 diet were significantly lower than those of the control (P < 0.05). In addition, the authors found that B. amyloliquefaciens LSG2-8 can regulate the intestinal flora balance and improve the intestinal structure of R. lagowskii. In conclusion, B. amyloliquefaciens LSG2-8 can improve the intestinal barrier function of R. lagowskii and can be used as a feed additive in aquaculture.
益生菌在水产养殖中被广泛应用。本文旨在研究解淀粉芽孢杆菌 LSG2-8 对瓦氏黄颡鱼肠道屏障功能的影响。将解淀粉芽孢杆菌 LSG2-8 添加到瓦氏黄颡鱼基础日粮（CK）中，作为添加剂，浓度分别为 1.0×10（D-6）、1.0×10（D-7）、1.0×10（D-8）和 1.0×10（D-9）CFU/g 基础日粮干重。经过 56 天的饲养试验，D-6、D-7、D-8 和 D-9 组瓦氏黄颡鱼肠道消化酶和免疫相关酶的活性均显著高于对照组（P<0.05）。在分子实验中，作者发现 D-8 组瓦氏黄颡鱼 TGF-β mRNA、IL-10 mRNA、ZO-1 mRNA 和 claudin-3 mRNA 的水平显著高于对照组和其他组（P<0.05）。此外，D-6、D-7、D-8 和 D-9 组瓦氏黄颡鱼的 IL-1β 和 IL-8 mRNA 水平均显著低于对照组（P<0.05）。此外，作者发现解淀粉芽孢杆菌 LSG2-8 可以调节肠道菌群平衡，改善瓦氏黄颡鱼的肠道结构。综上所述，解淀粉芽孢杆菌 LSG2-8 可以提高瓦氏黄颡鱼的肠道屏障功能，可作为水产养殖中的饲料添加剂。
