Thapa Damber, Samadi Nakisa, Tabatabaei Nima
Department of Mechanical EngineeringYork University Toronto ON M3J 1P3 Canada.
IEEE Sens J. 2021 Jun 14;21(17):18504-18511. doi: 10.1109/JSEN.2021.3089016. eCollection 2021 Sep 1.
With the emergence of vaccines and antibody therapeutics, rapid and scalable platforms are needed to quantify the antibody response of individuals. Lateral flow immunoassay (LFA) based test strips provide a rapid, low-cost, and point-of-care approach to antibody testing against the SARS-CoV-2 virus. These convenient and scalable tests, however, are qualitative in nature and cannot quantify the immune response of the infected and/or vaccinated individuals. This study reports on the development of a rapid, low cost and portable thermo-photonic device that enables sensitive detection and quantification of antibody levels using commercially available COVID-19 Antibody LFAs. Unlike conventional LFA readers, the developed technology is based on sensing the infrared thermal radiation of tag gold nanoparticles following laser excitation (aka photothermal response). Our proof-of-concept results with humanized monoclonal anti-SARS-CoV-2 Spike receptor-binding domain (RBD) IgG demonstrate that the thermo-photonic technology can detect and quantify antibody concentrations within the clinically relevant range and with a limit of detection of [Formula: see text]/ml. The reader in conjunction with antibody LFAs offers a low-cost, portable, and scalable solution for assessment of the degree of immunity in populations, quality control of convalescent plasma donations for antibody therapeutics, and monitoring the immune response of infected individuals and vaccine recipients.
随着疫苗和抗体疗法的出现,需要快速且可扩展的平台来量化个体的抗体反应。基于侧向流动免疫分析(LFA)的测试条为针对严重急性呼吸综合征冠状病毒2(SARS-CoV-2)病毒的抗体检测提供了一种快速、低成本且即时检测的方法。然而,这些便捷且可扩展的检测本质上是定性的,无法量化受感染和/或接种疫苗个体的免疫反应。本研究报告了一种快速、低成本且便携式的热光子设备的开发,该设备能够使用市售的新冠病毒抗体LFA灵敏地检测和量化抗体水平。与传统的LFA读数器不同,所开发的技术基于在激光激发后(即光热响应)感应标记金纳米颗粒的红外热辐射。我们使用人源化抗SARS-CoV-2刺突受体结合域(RBD)IgG的概念验证结果表明,热光子技术能够在临床相关范围内检测和量化抗体浓度,检测限为[公式:见原文]/ml。该读数器与抗体LFA相结合,为评估人群免疫程度、抗体疗法康复期血浆捐献的质量控制以及监测受感染个体和疫苗接种者的免疫反应提供了一种低成本、便携式且可扩展的解决方案。