Dey Biswadeep, Abraham Thangapalam Jawahar, Singha Jasmine, Roy Anwesha, Karmakar Sutanu, Kumar Patil Prasanna, Roy Utsa
Department of Aquatic Animal Health, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata, 700094 West Bengal India.
Department of Aquatic Environment Management, Faculty of Fishery Sciences, West Bengal University of Animal and Fishery Sciences, Chakgaria, Kolkata, 700094 West Bengal India.
Aquac Int. 2022;30(4):2113-2128. doi: 10.1007/s10499-022-00892-w. Epub 2022 May 13.
The current study evaluated the biosafety of oxytetracycline (OTC) exposure for 30 days in monosex fries. The fries were exposed to OTC for 3 h/day for 30 days at 350 (0.5X), 700 (1X), 2100 (3X), 3500 (5X), and 7000 (10X) mg/L and compared with control (0X). The OTC exposure at 5X and 10X concentrations caused 100% mortality within 4 days and 5 min, respectively. The mortalities recorded in 0.5X, 1X, and 3X groups were 3.33 ± 1.15%, 14.67 ± 1.15%, and 47.33 ± 11.37% on day 30, respectively. The feed intake was decreased up to 23.33% in the 3X group during the exposure period. The OTC residue levels on 30-day exposure were 216.53 ± 14.71, 450.56 ± 44.31, and 1141.26 ± 63.64 μg/kg, which reduced to 40.40 ± 3.25, 76.68 ± 2.77, and 95.61 ± 5.13 μg/kg after 15 days of termination of exposure in the 0.5X, 1X, and 3X groups, respectively. The histopathological changes observed in the 1X group were epithelial detachment, desquamation of secondary lamellar epithelium, lamellar fusion, and inflamed cartilaginous core in the gills, alteration in the integrity of gut mucosa, degeneration of muscularis mucosae and necrosis in the intestine, the disintegration of the nephritic tubule, necrosis, and glomerulopathy in the kidney, and dilated vascular duct, necrotized hepatic tissue, diffused hepatic parenchyma, vacuolation, and fatty changes in the liver. The OTC exposure induced marked tissue changes histologically in a dose- and time-dependent manner, which undoubtedly reduced the growth of tilapia.
The online version contains supplementary material available at 10.1007/s10499-022-00892-w.
本研究评估了土霉素（OTC）对单性鱼苗30天暴露的生物安全性。鱼苗每天暴露于OTC 3小时，持续30天，浓度分别为350（0.5倍）、700（1倍）、2100（3倍）、3500（5倍）和7000（10倍）mg/L，并与对照组（0倍）进行比较。5倍和10倍浓度的OTC暴露分别在4天和5分钟内导致100%的死亡率。在第30天，0.5倍、1倍和3倍组记录的死亡率分别为3.33±1.15%、14.67±1.15%和47.33±11.37%。在暴露期间，3倍组的采食量减少了23.33%。30天暴露后的OTC残留水平分别为216.53±14.71、450.56±44.31和1141.26±63.64μg/kg，在0.5倍、1倍和3倍组暴露终止15天后，分别降至40.40±3.25、76.68±2.77和95.61±5.13μg/kg。在1倍组观察到的组织病理学变化包括鳃上皮脱落、次生鳃小片上皮脱落、鳃小片融合和软骨核心发炎，肠道黏膜完整性改变，黏膜肌层变性和肠道坏死，肾小管解体、坏死和肾小球病变，以及血管导管扩张、肝组织坏死、肝实质弥漫性病变、空泡化和肝脏脂肪变性。OTC暴露在组织学上以剂量和时间依赖性方式诱导了明显的组织变化，这无疑降低了罗非鱼的生长。
在线版本包含可在10.1007/s10499-022-00892-w获取的补充材料。
