Choudhary Pushpa, Naik Saisweta P, Sahoo Sameer Ranjan, Das Rakesh, Sahoo Satya Narayan, Panda Satyen Kumar, Abraham Thangapalam Jawahar, Patil Prasanna Kumar, Swain Priyabrat, Mishra Sudhansu Sekhar
Indian Council of Agriculture Research (ICAR)-Central Institute of Freshwater Aquaculture (CIFA), Fish Health Management Division, Bhubaneswar, Odisha, India.
Fish Processing Division, Indian Council of Agriculture Research (ICAR)-Central Institute of Fisheries Technology, Cochin, Kerala, India.
Front Immunol. 2024 Dec 19;15:1478114. doi: 10.3389/fimmu.2024.1478114. eCollection 2024.
Aquaculture systems that sporadically depend on antibiotics can contribute to the development of adverse effects on the fish, microbial flora and the environment. This study sought to investigate the impacts of extended oxytetracycline supplementation on the freshwater stinging catfish through a multi-biomarker approach.
A total of 300 (20 ± 0.5 g) were placed in fibreglass-reinforced plastic tanks. The experimental fish were administered oxytetracycline hydrochloride (OTC) at varying doses, viz., 80 mg/kg fish biomass/day (1x), 240 mg (3x), 400 mg (5x), and 800 mg (10x) for 30 consecutive days. The study also included a control group that did not receive OTC.
OTC was effective against , , and , with minimum inhibitory concentrations ranging between 0.5 and 8.0 µg/mL. The OTC supplementation retarded the growth of fish. The respiratory burst activity, myeloperoxidase, and lysozyme increased significantly in the 1x group until day 20. This group showed an increase in serum albumin, whereas other OTC groups exhibited elevated levels of liver functional enzymes, including alanine transaminase, aspartate aminotransferase, and alkaline phosphatase. In addition, OTC groups exhibited increased levels of antioxidant enzymes. The magnitude of the increase was dose- and time-dependent. The liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) study signified a dose-dependent increase in OTC residues in the muscle. After a 10-day discontinuation of OTC, the tissue level retention of residues was minimal in the muscle, specifically in the 1x group compared to other groups. Significant histological alterations were noted in the liver tissues of the 5x and 10x groups, possibly due to oxidative stress and residue accretion.
The therapeutic dose of 80 mg/kg biomass/day was safe and tolerated well by , and may be used for sustainable catfish farming practices.
偶尔依赖抗生素的水产养殖系统可能会对鱼类、微生物群落和环境产生不良影响。本研究旨在通过多生物标志物方法研究延长土霉素补充对淡水刺鲶的影响。
将总共300条（20±0.5克）鱼放入玻璃纤维增强塑料水箱中。实验鱼连续30天接受不同剂量的盐酸土霉素（OTC），即80毫克/千克鱼体重/天（1倍剂量）、240毫克（3倍剂量）、400毫克（5倍剂量）和800毫克（10倍剂量）。该研究还包括一个未接受OTC的对照组。
OTC对[具体细菌名称1]、[具体细菌名称2]和[具体细菌名称3]有效，最低抑菌浓度在0.5至8.0微克/毫升之间。补充OTC会阻碍鱼类生长。在第20天之前，1倍剂量组的呼吸爆发活性、髓过氧化物酶和溶菌酶显著增加。该组血清白蛋白增加，而其他OTC组肝功能酶水平升高，包括丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶。此外，OTC组抗氧化酶水平升高。升高幅度呈剂量和时间依赖性。液相色谱-质谱/质谱（LC-MS/MS）研究表明肌肉中OTC残留量呈剂量依赖性增加。停止使用OTC 10天后，肌肉中残留组织水平的保留量最小，特别是1倍剂量组与其他组相比。在5倍剂量和10倍剂量组的肝脏组织中发现了明显的组织学改变，可能是由于氧化应激和残留积累。
80毫克/千克体重/天的治疗剂量对[具体鱼类名称]安全且耐受性良好，可用于可持续的鲶鱼养殖实践。
