Adekola Mukaila B, Areola Jacob O, Fagbohun Oladapo F, Asaolu Funke T, Ogundepo Gbenga E, Fajobi Adeniyi O, Babalola Olubunmi O
Department of Environmental Management and Toxicology, Federal University of Agriculture, Abeokuta, 110001, Nigeria.
Department of Medical Biochemistry, Obafemi Awolowo University, Ile-Ife, 230001, Nigeria.
J Pharm Anal. 2022 Apr;12(2):350-354. doi: 10.1016/j.jpha.2021.04.002. Epub 2021 Apr 10.
() K.D. Koenig (Family Sapindaceae) is a branchless straight bole approximately 15 m in length. The study evaluated the antioxidant and anti-inflammatory activities of ethanol extract and fractions of stem-bark using in vitro methods. Ethanol extract and its fractions were investigated for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), and quantitative phenolic and flavonoid contents. Anti-inflammatory activity was evaluated using albumin denaturation and membrane stabilization assays. The extract and its fractions exhibited radical scavenging and anti-inflammatory properties. The ethyl acetate fraction possessed maximum phenolic and flavonoid contents (136.67 ± 1.55 gallic acid equivalent mg/g and 75.76 ± 4.03 quercetin equivalent mg/g, respectively). Antioxidant studies revealed that the ethyl acetate fraction displayed superior activity with an IC = 0.09 ± 0.03 mg/mL DPPH, and values of 146.96 ± 3.81 ascorbic acid equivalent (AAE) mg/g and 359.20 ± 4.98 AAE mg/g for FRAP and TAC, respectively. Furthermore, the anti-inflammatory activity was revealed by inhibition of heat-induced albumin denaturation and red blood cell membrane stabilization at concentrations of 200-1000 μg/mL and 50-250 μg/mL, respectively. The ethanol extract and fractions exhibited antioxidant and anti-inflammatory activities, with ethyl acetate fraction showing superior activity, which could be attributed to secondary metabolites, mainly phenolic compounds. Overall, the antioxidant and anti-inflammatory activities of can be exploited by ethnomedicinal users.
（）无患子科的科氏倒地铃（K.D. Koenig）是一种无分支的直干植物，长度约为15米。该研究采用体外方法评估了其茎皮乙醇提取物及其馏分的抗氧化和抗炎活性。对乙醇提取物及其馏分进行了1,1-二苯基-2-苦基肼（DPPH）自由基清除活性、铁还原抗氧化能力（FRAP）、总抗氧化能力（TAC）以及酚类和黄酮类化合物含量的定量分析。通过白蛋白变性和膜稳定试验评估抗炎活性。提取物及其馏分具有自由基清除和抗炎特性。乙酸乙酯馏分的酚类和黄酮类化合物含量最高（分别为136.67±1.55没食子酸当量毫克/克和75.76±4.03槲皮素当量毫克/克）。抗氧化研究表明，乙酸乙酯馏分表现出优异的活性，DPPH的IC₅₀为0.09±0.03毫克/毫升，FRAP和TAC的值分别为146.96±3.81抗坏血酸当量（AAE）毫克/克和359.20±4.98 AAE毫克/克。此外，在浓度分别为200 - 1000微克/毫升和50 - 250微克/毫升时，通过抑制热诱导的白蛋白变性和红细胞膜稳定显示出抗炎活性。乙醇提取物及其馏分具有抗氧化和抗炎活性；乙酸乙酯馏分表现出优异的活性，这可能归因于次生代谢产物，主要是酚类化合物。总体而言，民族医药使用者可以利用其抗氧化和抗炎活性。
