从观赏鱼中分离出的分枝杆菌的抗生素敏感性
Antibiotic Susceptibility of Mycobacteria Isolated from Ornamental Fish.
作者信息
Guz Leszek, Puk Krzysztof
机构信息
Department of Biology and Fish Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland.
出版信息
J Vet Res. 2022 Mar 25;66(1):69-76. doi: 10.2478/jvetres-2022-0011. eCollection 2022 Mar.
INTRODUCTION
Nontuberculous mycobacteria (NTM) are increasingly recognised as causative agents of opportunistic infections in humans for which effective treatment is challenging. There is very little information on the prevalence of NTM drug resistance in Poland. This study was aimed to evaluate the susceptibility to antibiotics of NTM, originally isolated from diseased ornamental fish.
MATERIAL AND METHODS
A total of 99 isolates were studied, 50 of them rapidly growing mycobacteria (RGM) (among which three-quarters were , , and and the rest , , , , , , and ). The other 49 were slowly growing mycobacteria (SGM) isolates (among which only one was and the bulk and ). Minimum inhibitory concentrations for amikacin (AMK), kanamycin (KAN), tobramycin (TOB), doxycycline (DOX), ciprofloxacin (CIP), clarithromycin (CLR), sulfamethoxazole (SMX), isoniazid (INH) and rifampicin (RMP) were determined.
RESULTS
The majority of the isolates were susceptible to KAN (95.95%: RGM 46.46% and SGM 49.49%), AMK (94.94%: RGM 45.45% and SGM 49.49%), CLR (83.83%: RGM 36.36% and SGM 47.47%), SMX (79.79%: RGM 30.30% and SMG 49.49%), CIP (65.65%: RGM 24.24% and SGM 41.41%), and DOX (55.55%: RGM 9.06% and SGM 46.46%). The majority were resistant to INH (98.98%: RGM 50.50% and SGM 48.48%) and RMP (96.96%: RGM 50.50% and SGM 46.46%).
CONCLUSION
The drug sensitivity of NTM varies from species to species. KAN, AMK, CLR and SMX were the most active against RGM isolates, and these same four plus DOX and CIP were the best drugs against SGM isolates.
引言
非结核分枝杆菌（NTM）日益被认为是人类机会性感染的病原体，对其进行有效治疗具有挑战性。关于波兰NTM耐药性流行情况的信息非常少。本研究旨在评估最初从患病观赏鱼中分离出的NTM对抗生素的敏感性。
材料与方法
共研究了99株分离株，其中50株为快速生长分枝杆菌（RGM）（其中四分之三为 、 和 ，其余为 、 、 、 、 、 ）。另外49株为缓慢生长分枝杆菌（SGM）分离株（其中只有1株为 ，大部分为 和 ）。测定了阿米卡星（AMK）、卡那霉素（KAN）、妥布霉素（TOB）、多西环素（DOX）、环丙沙星（CIP）、克拉霉素（CLR）、磺胺甲恶唑（SMX）、异烟肼（INH）和利福平（RMP）的最低抑菌浓度。
结果
大多数分离株对KAN（95.95%：RGM为46.46%，SGM为49.49%）、AMK（94.94%：RGM为45.45%，SGM为49.49%）、CLR（83.83%：RGM为36.36%，SGM为47.47%）、SMX（79.79%：RGM为30.30%，SMG为49.49%）、CIP（65.65%：RGM为24.24%，SGM为41.41%）和DOX（55.55%：RGM为9.06%，SGM为46.46%）敏感。大多数分离株对INH（98.98%：RGM为50.50%，SGM为48.48%）和RMP（96.96%：RGM为50.50%，SGM为46.46%）耐药。
结论
NTM的药敏情况因菌种而异。KAN、AMK、CLR和SMX对RGM分离株活性最强，这四种药物加上DOX和CIP对SGM分离株效果最佳。
Zotero 插件