Microbiome Network and Department of Agricultural Biology, Colorado State University, Fort Collins, CO.
Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.
Environ Microbiol. 2022 Aug;24(8):3722-3734. doi: 10.1111/1462-2920.16031. Epub 2022 May 17.
The frequency and severity of drought are increasing due to anthropogenic climate change and are already limiting cropping system productivity in many regions around the world. Few microbial groups within plant microbiomes can potentially contribute towards the fitness and productivity of their hosts under abiotic stress events including water deficits. However, microbial communities are complex and integrative work considering the multiple co-existing groups of organisms is needed to better understand how the entire microbiome responds to environmental stresses. We hypothesize that water deficit stress will differentially shape bacterial, fungal, and protistan microbiome composition and influence inter-kingdom microbial interactions in the rhizospheres of corn and sugar beet. We used amplicon sequencing to profile bacterial, fungal, and protistan communities in corn and sugar beet rhizospheres grown under irrigated and water deficit conditions. The water deficit treatment had a stronger influence than host species on bacterial composition, whereas the opposite was true for protists. These results indicate that different microbial kingdoms have variable responses to environmental stress and host factors. Water deficit also influenced intra- and inter-kingdom microbial associations, wherein the protist taxa formed a separate cluster under water deficit conditions. Our findings help elucidate the influence of environmental and host drivers of bacterial, fungal, and protistan community assembly and co-occurrence in agricultural rhizosphere environments.
由于人为气候变化，干旱的频率和严重程度正在增加，这已经限制了世界许多地区的作物系统生产力。在包括水分亏缺在内的非生物胁迫事件下，植物微生物组中的少数微生物群可能有助于其宿主的适应性和生产力。然而，微生物群落是复杂的，需要综合考虑多个共存的生物体群体的工作，以更好地了解整个微生物组如何对环境压力做出反应。我们假设水分亏缺胁迫将差异地塑造细菌、真菌和原生动物微生物组组成，并影响玉米和甜菜根际的微生物间相互作用。我们使用扩增子测序来分析在灌溉和水分亏缺条件下生长的玉米和甜菜根际的细菌、真菌和原生动物群落。水分亏缺处理对细菌组成的影响大于宿主物种，而对于原生动物则相反。这些结果表明，不同的微生物王国对环境压力和宿主因素有不同的反应。水分亏缺还影响了微生物的种内和种间相互作用，其中在水分亏缺条件下，原生动物类群形成了一个单独的聚类。我们的研究结果有助于阐明环境和宿主驱动细菌、真菌和原生动物群落组装和共存的影响，在农业根际环境中。
