A. A. Jaczewskii Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection, Shosse Podbelskogo 3, Pushkin, Saint Petersburg, 196608, Russia.
Mycologia. 2022 May-Jun;114(3):556-574. doi: 10.1080/00275514.2022.2040285. Epub 2022 May 18.
Phomopsis stem canker is economically important sunflower disease that caused by multiple species. Recent investigations resulted in the resolution that there are at least 13 species that can infect sunflower. A comprehensive analysis of the biodiversity and geographic distribution of species in Russia, particularly those that infect sunflower, has not been undertaken. For this study, 16 isolates were obtained from samples of stem canker and visually healthy seeds of from northwestern, central European, southern European Russia, North Caucasus, and the Urals in 2016-2019. The aim of this study was to identify these isolates based on morphology and sequence analyses of the nuclear ribosomal internal transcribed spacer (ITS) region, partial calmodulin (), DNA-lyase (), histone H3 (), translation elongation factor-1α gene (), and ß-tubulin () genes. The phylogenetic reconstruction revealed well-supported monophyletic clades corresponding to six species: , and . Two new species were described: sp. nov. and sp. nov. The isolates of and collected represent the first records of these species in Russia.
茎基溃疡病是一种重要的向日葵病害，由多种病原菌引起。最近的研究结果表明，至少有 13 种病原菌可以感染向日葵。然而，针对俄罗斯病原菌的生物多样性和地理分布，特别是那些感染向日葵的病原菌，尚未进行全面分析。在这项研究中，我们于 2016 年至 2019 年，从来自俄罗斯西北部、中欧、南欧、北高加索和乌拉尔地区的茎基溃疡病和外观健康的向日葵种子样本中，获得了 16 个分离株。本研究的目的是基于核核糖体内部转录间隔区（ITS）区域、部分钙调蛋白（）、DNA 连接酶（）、组蛋白 H3（）、翻译延伸因子 1α 基因（）和 β-微管蛋白（）基因的形态和序列分析，对这些分离株进行鉴定。系统发育重建显示，与 6 种病原菌属：、、、、和 相对应的单系分支得到了很好的支持。描述了两个新种：和 。本研究中收集到的 和 的分离株代表了这些病原菌在俄罗斯的首次记录。
