Department of Environmental Studies, Satyawati College, University of Delhi, Delhi, 110052, India.
Department of Chemical Engineering, Birla Institute of Technology, Mesra, Ranchi 835215, Jharkhand, India.
Sci Total Environ. 2022 Sep 10;838(Pt 1):155966. doi: 10.1016/j.scitotenv.2022.155966. Epub 2022 May 15.
Sustainable and practically viable biofuels production technology using lignocellulosic biomass is still seeking its way of implementation owing to some major issues involved therein. Unavailability of efficient microbial sources for the degradation of cellulosic biomass is one of the major roadblocks in biomass to biofuels production technology. In this context, utilization of microbiomes to degrade lignocellulaosic biomass is emerging as a rapid and effective approach that can fulfill the requirements of biomass based biofuels production technology. Therefore, the present review is targeted to explore soil metagenomic approach to improve the lignocellulosic biomass degradation processing for the cost-effective and eco-friendly application. Soil microbiomes consist of rich microbial community along with high probability of cellulolytic microbes, and can be identified by culture independent metagenomics method which can be structurally and functionally explored via genomic library. Therefore, in depth analysis and discussion have also been made via structural & functional metagenomics tools along with their contribution to genomic library. Additionally, the present review highlights currently existing bottlenecks along with their feasible solutions. This review will help to understand the basic research as well as industrial concept for the process improvement based on soil microbiome mediated lignocellulosic biomass degradation, and this may likely to implement for the low-cost commercial biofuels production technology.
利用木质纤维素生物质生产可持续且可行的生物燃料技术仍在探索实施途径，因为其中涉及一些重大问题。缺乏用于纤维素生物质降解的高效微生物源是生物质向生物燃料生产技术的主要障碍之一。在这种情况下，利用微生物组来降解木质纤维素生物质作为一种快速有效的方法正在兴起，它可以满足基于生物质的生物燃料生产技术的要求。因此，本综述旨在探索土壤宏基因组方法，以提高木质纤维素生物质的降解处理效率，实现经济实惠和环保的应用。土壤微生物组包含丰富的微生物群落，同时也有很高的可能存在纤维素分解微生物，可以通过非培养的宏基因组学方法来识别，该方法可以通过基因组文库进行结构和功能探索。因此，还通过结构和功能宏基因组学工具对其进行了深入分析和讨论，并讨论了它们对基因组文库的贡献。此外，本综述还强调了目前存在的瓶颈及其可行的解决方案。本综述将有助于理解基于土壤微生物组介导的木质纤维素生物质降解的基础研究和工业概念，这可能有助于实施低成本的商业生物燃料生产技术。
