Eastern Theater Command Centers for Disease Prevention and Control, Nanjing, China.
School of Medicine, Jiangsu University, Zhenjiang, China.
J Vet Med Sci. 2022 Jun 17;84(6):847-854. doi: 10.1292/jvms.22-0058. Epub 2022 May 17.
Ticks are an important group of arthropod vectors. Ticks pose a profound risk to public health by transmitting many types of microorganisms that are human and animal pathogens. With the development of next-generation sequencing (NGS) technology and viral metagenomics, numerous novel viruses have been discovered in ticks and tick-related hosts. To fully understand the virus spectrum in ticks in the Zhoushan Archipelago of Zhejiang province in China, ticks were collected from Qushan Island, Zhoushan Island, and Daishan Island in the Zhoushan Archipelago in June 2016. NGS performed to investigate the diversity of tick-associated viruses identified 21 viral sequences. Twelve were pathogenic to humans and animals. Trough verification by polymerase chain reaction (PCR) revealed the existence of three tick-associated viruses with extensive homology with Dabieshan, MG22, and Odaw virus. Other NGS-detected sequences that could not be amplified by PCR were highly homologous (92-100%) with known pathogenic viruses that included hepatitis B virus, papillomavirus, and human mastadenovirus C. This is the first study to systematically apply high throughput sequencing technology to explore the spectrum of viruses carried by ticks in the Zhoushan Archipelago. The findings are fundamental knowledge of the diversity of tick-associated viruses in this region and will inform strategies to monitor and prevent the spread of tick-borne diseases.
蜱虫是一种重要的节肢动物媒介。蜱虫通过传播多种人类和动物病原体微生物，对公共卫生构成了严重威胁。随着下一代测序（NGS）技术和病毒宏基因组学的发展，在蜱虫和蜱虫相关宿主中发现了许多新型病毒。为了全面了解中国浙江省舟山群岛的蜱虫中的病毒谱，我们于 2016 年 6 月从舟山群岛的衢山岛、舟山岛和岱山岛采集了蜱虫。通过 NGS 对蜱虫相关病毒的多样性进行调查，共鉴定出 21 条病毒序列。其中 12 条对人类和动物具有致病性。通过聚合酶链反应（PCR）验证，发现存在三种与大别山、MG22 和 Odaw 病毒具有广泛同源性的蜱虫相关病毒。其他无法通过 PCR 扩增的 NGS 检测到的序列与已知的致病性病毒高度同源（92-100%），包括乙型肝炎病毒、乳头瘤病毒和人巨细胞病毒 C。这是首次系统地应用高通量测序技术来探索舟山群岛蜱虫携带的病毒谱的研究。这些发现是该地区蜱虫相关病毒多样性的基础知识，将为监测和预防蜱传疾病的传播提供策略。
