Su Si, Cui Meng-Yu, Xing Li-Li, Gao Rui-Juan, Mu Lan, Hong Mei, Guo Qi-Qi, Ren Hong, Yu Jing-Feng, Si Xiao-Yan, Eerde Mutu
Graduate School, Inner Mongolia Medical University, Hohhot, Inner Mongolia, China.
Department of Pathology, Yueyang Central Hospital, Yueyang, Hunan, China.
PLoS Negl Trop Dis. 2024 Dec 11;18(12):e0012706. doi: 10.1371/journal.pntd.0012706. eCollection 2024 Dec.
Ticks are widely distributed throughout China and are the second most prevalent pathogen vectors in the world, following only mosquitoes. Tick bites can lead to Lyme disease, forest encephalitis, and other illnesses that may result in death under severe circumstances. Materials and methods: Ticks collected from March 2021 to May 2023 were pooled and used in metatranscriptomic analyses to gain insight into the diversity and distribution of tick-borne viruses in Inner Mongolia. Next-generation sequencing (NGS) outcomes were validated, and viral prevalence across distinct tick species was determined through the application of polymerase chain reaction (PCR) paired with Sanger sequencing.
A total of 20 RNA viruses belonging to at least 8 families, including Chuviridae, Flaviviridae, Solemoviridae, Nairoviridae, Partitiviridae, Phenuiviridae, Rhabdoviridae, and Totiviridae, and to unclassified families were identified by NGS. Five of the identified RNA viruses (Nuomin virus, Yezo virus, tick-borne encephalitis virus, Alongshan virus, and Beiji nairovirus) are considered human pathogens. A potential human pathogen, Mukawa virus, was also among the identified viruses. Ixodes persulcatus carried a significantly greater number of viral species than did Dermacentor nuttalli, Hyalomma marginatum, and Haemaphysalis concinna. The prevalence of coinfection with multiple viruses differed in I. persulcatus from Hinggan League and Hulun Buir, and Beiji nairovirus was the codominant virus species.
There is a remarkable diversity of RNA viruses harboured by ticks in Inner Mongolia, with variations observed in the distribution of these tick-borne viruses across different regions and tick hosts.
蜱在中国分布广泛，是世界上第二大最普遍的病原体传播媒介，仅次于蚊子。蜱叮咬可导致莱姆病、森林脑炎和其他疾病，在严重情况下可能导致死亡。材料与方法：收集2021年3月至2023年5月的蜱，合并后用于宏转录组分析，以深入了解内蒙古蜱传病毒的多样性和分布。对下一代测序（NGS）结果进行验证，并通过聚合酶链反应（PCR）结合桑格测序确定不同蜱种的病毒流行情况。
通过NGS鉴定出至少8个科的20种RNA病毒，包括楚病毒科、黄病毒科、索勒莫病毒科、内罗病毒科、双分病毒科、白蛉病毒科、弹状病毒科和 Totiviridae科以及未分类的科。鉴定出的RNA病毒中有5种（诺敏病毒、耶佐病毒、蜱传脑炎病毒、阿龙山病毒和北极内罗病毒）被认为是人类病原体。一种潜在的人类病原体穆卡瓦病毒也在鉴定出的病毒中。全沟硬蜱携带的病毒种类明显多于边缘革蜱、纳氏革蜱和嗜群血蜱。兴安盟和呼伦贝尔的全沟硬蜱中多种病毒共感染的流行情况不同，北极内罗病毒是共同优势病毒种类。
内蒙古蜱携带的RNA病毒种类繁多，这些蜱传病毒在不同地区和蜱宿主中的分布存在差异。
