School of Public Health, WeiFang Medical University, 261053, China.
Tianjin International Joint Academy of Biomedicine, Tianjin, 300457, China.
J Infect. 2020 Dec;81(6):973-978. doi: 10.1016/j.jinf.2020.10.022. Epub 2020 Oct 25.
The aim of this study is to systematically examine the variety of viruses maintained in ticks from Shandong Province. A total of 2522 ticks were sampled from five cities of Shandong Province and divided into 264 pools according to location and species. Viral megagenomic analysis revealed the sequences of two viruses, Dabieshan tick virus and SFTSV. Then qRT-PCR and nested PCR were performed to confirm the presence of corresponding pathogens, which showed positive results for Dabieshan tick virus and SFTSV, with a minimum infection rate of 0.67% (17/2522) and 2.5% (63/2522), respectively. Phylogenetic analysis showed that Dabieshan tick virus formed a monophyletic cluster with the Yongjia tick virus and Uukuniemi virus from China, and SFTSV shared over 95% identity with human and animal derived isolates. These findings are the first time to demonstrate molecular evidence of Dabieshan tick virus in unrecognized endemic regions and indicate the need for further investigation.
本研究旨在系统研究山东省蜱虫中携带的多种病毒。共从山东省五个城市采集了 2522 只蜱虫，根据地点和种类分为 264 个池。病毒宏基因组分析揭示了两种病毒，大别山蜱病毒和 SFTSV 的序列。然后进行 qRT-PCR 和巢式 PCR 以确认相应病原体的存在，结果显示大别山蜱病毒和 SFTSV 均为阳性，最低感染率分别为 0.67%（17/2522）和 2.5%（63/2522）。系统进化分析表明，大别山蜱病毒与中国的永嘉蜱病毒和乌库尼米病毒形成单系群，SFTSV 与人及动物来源的分离株的同源性超过 95%。这些发现首次证明了大别山蜱病毒在未被识别的地方性流行地区的分子证据，表明需要进一步调查。
