Plachot M, Junca A M, Mandelbaum J, Cohen J, Salat-Baroux J, Da Lage C
Hum Reprod. 1986 Jun;1(4):237-42. doi: 10.1093/oxfordjournals.humrep.a136392.
In humans, in contrast to other species, sperm capacitation requires a very short time, as in-vitro fertilization has been obtained after only 45 min of contact between oocytes and spermatozoa capacitated for 1 h. No fertilization occurred, whatever the duration of sperm capacitation, when gamete mixing did not exceed 30 min. On the contrary, 85% of cumulus-free mature oocytes exposed to sperm for 1-4 h were fertilized. The presence of the pre-ovulatory, fully-expanded or compact cumulus mass did not represent a physical barrier to sperm progression, as we observed no delay in fertilization when oocytes were enclosed in the cumulus. The use of a short insemination protocol (1-4 h instead of 17-20 h) did not reduce the fertilization rate of denuded or cumulus-enclosed oocytes and had no significant effect on the morphological appearance of the embryos or their cleavage rates.
与其他物种不同，在人类中，精子获能所需时间非常短，因为在卵母细胞与获能1小时的精子仅接触45分钟后就实现了体外受精。当配子混合时间不超过30分钟时，无论精子获能的持续时间如何，均未发生受精。相反，暴露于精子1 - 4小时的85%无卵丘成熟卵母细胞受精。排卵前充分扩张或紧密的卵丘团块的存在并不构成精子前进的物理障碍，因为当卵母细胞被包裹在卵丘中时，我们未观察到受精延迟。采用短授精方案（1 - 4小时而非17 - 20小时）并未降低裸卵或有卵丘包裹卵母细胞的受精率，对胚胎的形态外观或其分裂率也没有显著影响。
