体外受精期间短时间配子共孵育会降低受精率且不会改善胚胎质量：一项前瞻性自身对照研究。
Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality: a prospective auto controlled study.
作者信息
Barraud-Lange V, Sifer C, Pocaté K, Ziyyat A, Martin-Pont B, Porcher R, Hugues J N, Wolf J P
机构信息
Service d'Histologie Embryologie Cytogénétique, Hôpital Jean Verdier, Avenue du 14 Juillet, 93140 Bondy, France.
出版信息
J Assist Reprod Genet. 2008 Jul;25(7):305-10. doi: 10.1007/s10815-008-9240-3. Epub 2008 Aug 1.
Abstract
PURPOSE
Evaluate the effect of short gamete incubation on fertilization rate and embryo quality.
METHODS
A prospective study has been performed. Two thousand five hundred and forty seven sibling oocytes from 240 couples undergoing IVF attempts were allocated to a short (1 h) or a standard (18 h) insemination procedure. Diploid fertilization rate (two pronuclei, 2PN), polyspermy (>2PN) and embryo quality were compared.
RESULTS
The fertilization rate was statistically lower in the short insemination group compared to the standard insemination one (64.9% and 70.1%; P = 0.039), with a similar polyspermy rate observed between the two groups. A slight, but non significant, increase was observed concerning good embryo quality rate in the short insemination group when compared to the standard insemination, both at day 2 (60.1 vs. 58.1%; P = 0.06) and day 3 (53.2 vs. 48.5%; P = 0.22).
CONCLUSION
This new study highlights that a 1 h gamete exposure decreases the fertilization rate and does not improve embryo quality compared with a standard 18 h insemination procedure.
摘要
目的
评估配子短时间孵育对受精率和胚胎质量的影响。
方法
进行了一项前瞻性研究。将240对接受体外受精尝试的夫妇的2547个同胞卵母细胞分配到短时间（1小时）或标准（18小时）授精程序中。比较二倍体受精率（双原核，2PN）、多精受精（>2PN）和胚胎质量。
结果
与标准授精组相比，短时间授精组的受精率在统计学上较低（分别为64.9%和70.1%；P = 0.039），两组的多精受精率相似。与标准授精组相比，短时间授精组在第2天（60.1%对58.1%；P = 0.06）和第3天（53.2%对48.5%；P = 0.22）时，优质胚胎率有轻微但不显著的增加。
结论
这项新研究表明，与标准的18小时授精程序相比，1小时的配子暴露会降低受精率且不会改善胚胎质量。
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