Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing, China.
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Beijing, China.
PLoS One. 2022 May 19;17(5):e0268373. doi: 10.1371/journal.pone.0268373. eCollection 2022.
Helicobacter pylori (H. pylori) encodes numerous outer membrane proteins (OMPs), with considerable geographic heterogeneity and related to different clinical outcomes. This study aimed to investigate the distribution characteristics of five important OMP genes (sabA, hofC, homA, homB and frpB-4) in different regions of China.
A total of 266 strains were isolated from 348 stomach biopsy specimens in Shandong, Guangxi, Heilongjiang, Hunan, and Qinghai provinces. The presence of sabA, hofC, homA, homB and frpB-4 gene was detected by polymerase chain reaction (PCR) from H. pylori genomic DNA.
Among the strains in five regions, the prevalence of frpB-4 was 100% and that of hofC was 97.7%. The prevalence of homB in the isolates from Qinghai (45.5%) was significantly lower than that in Shandong (75.3%), Guangxi (76.9%) and Hunan (69.6%) (P<0.05). The frequency of homA in Shandong (30.1%) was significantly lower than in Guangxi (57.7%) and Qinghai (63.6%) (P<0.05). The prevalence of the sabA gene in Shandong, Guangxi, Heilongjiang, Hunan and Qinghai provinces was 21.9%, 59.7%, 45.9%, 52.2%, and 18.2%, respectively (P<0.05). The sabA "on" status was significantly more frequent in isolates from Guangxi (46.8%), Heilongjiang (37.8%), and Hunan (47.8%) than Qinghai (3.0%) (P<0.05). The presence of homA and sabA genes may be negatively correlated with the development of gastritis. There was no significant association between the frpB-4, hofC, homB gene and clinical outcomes.
The prevalence of homA, homB, and sabA genes and the sabA "on" or "off" status have significant geographical differences among five provinces in China. The presence of homA and sabA genes may be protective factors of gastritis.
幽门螺杆菌（H. pylori）编码多种外膜蛋白（OMPs），具有显著的地理异质性，与不同的临床结局相关。本研究旨在探讨中国不同地区五种重要的 OMP 基因（sabA、hofC、homA、homB 和 frpB-4）的分布特征。
从山东、广西、黑龙江、湖南和青海的 348 例胃活检标本中分离出 266 株菌。应用聚合酶链反应（PCR）从 H. pylori 基因组 DNA 中检测 sabA、hofC、homA、homB 和 frpB-4 基因的存在。
在五个地区的菌株中，frpB-4 的流行率为 100%，hofC 的流行率为 97.7%。青海分离株中 homB 的流行率（45.5%）明显低于山东（75.3%）、广西（76.9%）和湖南（69.6%）（P<0.05）。山东分离株中 homA 的频率（30.1%）明显低于广西（57.7%）和青海（63.6%）（P<0.05）。山东、广西、黑龙江、湖南和青海的 sabA 基因流行率分别为 21.9%、59.7%、45.9%、52.2%和 18.2%（P<0.05）。广西（46.8%）、黑龙江（37.8%）和湖南（47.8%）分离株的 sabA“on”状态明显高于青海（3.0%）（P<0.05）。homA 和 sabA 基因的存在可能与胃炎的发生呈负相关。frpB-4、hofC、homB 基因与临床结局之间无显著相关性。
中国五个省份的 homA、homB 和 sabA 基因的流行率以及 sabA“on”或“off”状态存在显著的地域差异。homA 和 sabA 基因的存在可能是胃炎的保护因素。
