Zhao Qiaoyun, Song Conghua, Wang Ke, Li Donghong, Yang Yang, Liu Dongsheng, Wang Le, Zhou Nanjin, Xie Yong
Department of Gastroenterology, First Affiliated Hospital of Nanchang University, No.17 Yongwaizheng Street, Donghu District, Nanchang, 330006, Jiangxi, China.
Microbiology Laboratory, Gastroenterology Institute of Jiangxi Province, Nanchang, 330006, Jiangxi, China.
Med Microbiol Immunol. 2020 Oct;209(5):565-577. doi: 10.1007/s00430-020-00666-2. Epub 2020 Mar 26.
Disease outcome is associated with virulence factors of Helicobacter pylori (H. pylori), which are partially attributed to the outer membrane protein (OMP). This study aimed to investigate the correlation between the four OMP genes (babA, oipA, sabA, and homB) and gastroduodenal diseases. One hundred and seventy-seven H. pylori strains were isolated from Chinese patients with different gastroduodenal diseases (49 chronic gastritis, 19 gastric ulcer, 33 gastric cancer, and 76 duodenal ulcer), 94 of which contained pathological information (41 superficial gastritis, 24 intestinal hyperplasia, and 29 gastric adenocarcinoma). The full-length amplification of babA, oipA, sabA, and homB genes was acquired and sequenced. Then, the genetic polymorphism was analyzed to compare with the reference strains from the GenBank database. Functional status and cluster analysis were also performed to evaluate the impact of genetic polymorphism on disease outcome. The prevalence of babA, oipA, sabA, and homB genes were 91.5%, 100%, 94.0%, and 95.5%, respectively. The four OMP genes were characterized by genetic polymorphism and in the status of positive selection (Ka/Ks> 1). The proportion of strains with functional status on for oipA and sabA gene was 100% and 76.2%, respectively. The sequences of four OMP genes were mainly clustered together with the East Asian references. The four OMP genes were not different in patients with gastroduodenal diseases and pathologic changes (P > 0.05). H. pylori babA, oipA, sabA, and homB genes were common in the Chinese populations, but did not seem to be involved in the development of gastroduodenal diseases.
疾病结局与幽门螺杆菌（H. pylori）的毒力因子相关，其中部分归因于外膜蛋白（OMP）。本研究旨在探讨四种OMP基因（babA、oipA、sabA和homB）与胃十二指肠疾病之间的相关性。从患有不同胃十二指肠疾病的中国患者中分离出177株幽门螺杆菌（49例慢性胃炎、19例胃溃疡、33例胃癌和76例十二指肠溃疡），其中94株包含病理信息（41例浅表性胃炎、24例肠化生和29例胃腺癌）。获得了babA、oipA、sabA和homB基因的全长扩增产物并进行测序。然后，分析基因多态性并与GenBank数据库中的参考菌株进行比较。还进行了功能状态和聚类分析，以评估基因多态性对疾病结局的影响。babA、oipA、sabA和homB基因的流行率分别为91.5%、100%、94.0%和95.5%。这四种OMP基因具有基因多态性且处于正选择状态（Ka/Ks>1）。oipA和sabA基因功能状态为阳性的菌株比例分别为100%和76.2%。四种OMP基因的序列主要与东亚参考菌株聚类在一起。四种OMP基因在胃十二指肠疾病患者和病理变化中无差异（P>0.05）。幽门螺杆菌的babA、oipA、sabA和homB基因在中国人群中很常见，但似乎不参与胃十二指肠疾病的发生发展。
