School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China.
Department of Hepatology, Fifth Medical Center of Chinese PLA General Hospital, Beijing, China.
Pharm Biol. 2022 Dec;60(1):958-967. doi: 10.1080/13880209.2022.2067569.
(Turcz.) Baill. (Magnoliaceae) essential oil (SCEO) composition is rich in lignans that are believed to perform protective effects in the liver.
This study investigates the effects of SCEO in the treatment of acetaminophen (APAP)-induced liver injury in mice.
C57BL/6 mice ( = 56) were randomly divided into seven groups: normal; APAP (300 mg/kg); APAP plus bicyclol (200 mg/kg); APAP plus SCEO (0.25, 0.5, 1, 2 g/kg). Serum biochemical parameters for liver function, inflammatory factors, and antioxidant activities were determined. The protein expression levels of Nrf2, GCLC, GCLM, HO-1, p62, and LC3 were assessed by western blotting. Nrf2, GCLC, HO-1, p62, and LC3 mRNA were detected by real-time PCR.
Compared to APAP overdose, SCEO (2 g/kg) pre-treatment reduced the serum levels of AST (79.4%), ALT (84.6%), TNF-α (57.3%), and IL-6 (53.0%). In addition, SCEO (2 g/kg) markedly suppressed cytochrome P450 2E1 (CYP2E1) (15.4%) and attenuated the exhaustion of GSH (43.6%) and SOD (16.8%), and the accumulation of MDA (22.6%) in the liver, to inhibit the occurrence of oxidative stress. Moreover, hepatic tissues from our experiment revealed that SCEO pre-treatment mitigated liver injury caused by oxidative stress by increasing Nrf2, HO-1, and GCL. Additionally, SCEO activated autophagy, which upregulated hepatic LC3-II and decreased p62 in APAP overdose mice ( < 0.05).
Our evidence demonstrated that SCEO protects hepatocytes from APAP-induced liver injury and the findings will provide a reliable theoretical basis for developing novel therapeutics.
（Turcz.）Baill.（木兰科）精油（SCEO）的成分富含木脂素，据信这些木脂素对肝脏具有保护作用。
本研究旨在探讨 SCEO 对乙酰氨基酚（APAP）诱导的小鼠肝损伤的治疗作用。
将 56 只 C57BL/6 小鼠随机分为 7 组：正常组；APAP（300mg/kg）组；APAP+双环醇（200mg/kg）组；APAP+SCEO（0.25、0.5、1、2g/kg）组。测定血清肝功能生化参数、炎症因子和抗氧化活性。通过蛋白质印迹法检测 Nrf2、GCLC、GCLM、HO-1、p62 和 LC3 的蛋白表达水平。通过实时 PCR 检测 Nrf2、GCLC、HO-1、p62 和 LC3 的 mRNA 表达。
与 APAP 过量相比，SCEO（2g/kg）预处理降低了血清 AST（79.4%）、ALT（84.6%）、TNF-α（57.3%）和 IL-6（53.0%）水平。此外，SCEO（2g/kg）显著抑制细胞色素 P450 2E1（CYP2E1）（15.4%），减轻 GSH（43.6%）和 SOD（16.8%）的耗竭，以及 MDA（22.6%）在肝脏中的积累，抑制氧化应激的发生。此外，实验性肝组织表明，SCEO 预处理通过增加 Nrf2、HO-1 和 GCL 来减轻氧化应激引起的肝损伤。此外，SCEO 激活自噬，增加 APAP 过量小鼠肝 LC3-II，减少 p62（<0.05）。
我们的证据表明，SCEO 可保护肝细胞免受 APAP 引起的肝损伤，为开发新型治疗药物提供了可靠的理论依据。
