Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795 Cairo, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Helwan University, Ein Helwan, 11795 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121388. doi: 10.1016/j.saa.2022.121388. Epub 2022 May 14.
In this work, a new and simple carbon dots (CDs) based fluorescent probe was introduced for selective determination of diacerein (DIA) in presence of two co-formulated drugs. This highly fluorescent sensor was constructed using chitosan as a carbon and nitrogen source by single step carbonization. The constructed probe is based on the inner filter effect (IFE), in which DIA serves as a strong absorber, influencing the excitation of the fluorescer (CDs). This overlap leads to quenching of CDs fluorescence upon increasing DIA concentration within the range of 2.5-17.5 µg/mL with mean % recovery reached to 99.7 ± 0.7. The performance of the constructed sensor had been validated according to the ICH guidelines and the results revealed that it is precise and accurate. Moreover, it has many advantages such as simplicity, saving time and good selectivity for the determination of DIA as a minor component in presence of co-formulated drugs in its tablet dosage form.
在这项工作中，引入了一种新的简单的基于碳点（CDs）的荧光探针，用于在两种共同配方药物存在的情况下选择性测定双醋瑞因（DIA）。该高荧光传感器是通过一步碳化法使用壳聚糖作为碳和氮源构建的。所构建的探针基于内滤效应（IFE），其中 DIA 作为强吸收剂，影响荧光团（CDs）的激发。这种重叠导致在 2.5-17.5 µg/mL 的范围内随着 DIA 浓度的增加，CDs 荧光猝灭，平均回收率达到 99.7±0.7。根据 ICH 指南验证了所构建传感器的性能，结果表明它具有精确性和准确性。此外，它具有许多优点，例如简单、省时以及在片剂制剂中存在共同配方药物的情况下作为次要成分测定 DIA 时具有良好的选择性。
