Department of Analytical Chemistry, Faculty of Pharmacy, Helwan University, Helwan, 11795 Cairo, Egypt.
Anal Methods. 2024 Sep 26;16(37):6373-6382. doi: 10.1039/d4ay01216j.
In this study, a new, rapid, simple, and cost-effective fluorescent probe based on carbon dots (CDs) has been developed for the selective determination of nitrazepam (NZP). The CDs were synthesized with high production yield (71.43%) through one step carbonization of dried banana peels. The fluorescence intensity of the CDs is quenched by increasing the NZP concentration as a result of the inner-filter effect and dynamic quenching mechanism. This fluorescent sensor effectively quantified NZP in the linear range of 0.30-26 μg mL, with a limit of detection of 0.10 μg mL. The performance of the sensor was validated according to ICH guidelines and exhibited high precision and accuracy. The proposed method was also successfully extended for determining NZP in tablet dosage form and milk samples.
在这项研究中，开发了一种新的、快速、简单且经济高效的基于碳点 (CDs) 的荧光探针，用于选择性测定硝基安定 (NZP)。通过一步碳化干燥的香蕉皮，以 71.43%的高产率合成了 CDs。随着 NZP 浓度的增加，由于内滤效应和动态猝灭机制，CDs 的荧光强度被猝灭。这种荧光传感器可以在 0.30-26 μg mL 的线性范围内有效定量 NZP，检测限为 0.10 μg mL。根据 ICH 指南验证了传感器的性能，表现出高的精密度和准确度。该方法还成功扩展到片剂制剂和牛奶样品中测定 NZP。
