Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
Pharmaceutical Services Center, Faculty of Pharmacy, Tanta University, Tanta, 31527, Egypt.
J Fluoresc. 2021 May;31(3):675-684. doi: 10.1007/s10895-021-02698-0. Epub 2021 Feb 10.
In this study, a novel and simple fluorescent carbon quantum dots (CQDs) based nano-sensor for colchicine determination has been prepared. The nitrogen doped CQDs probe was prepared using uric acid as a carbon/nitrogen source via a one-step pyrolysis. The sensor is based on inner filter effect (IFE) where colchicine acts as a powerful absorber that affects the excitation of the fluorescer (CQDs). This overlap results in a quantitative attenuation of the fluorescence of CQDs with increasing colchicine concentration in the range of 2-25 μM. The developed sensor has the advantages of simplicity, less time-consuming, convenience and satisfactory selectivity for colchicine determination in pharmaceutical dosage forms.
本研究制备了一种新颖且简单的基于荧光碳量子点（CQDs）的纳传感器，用于秋水仙碱的测定。氮掺杂 CQDs 探针是通过一步热解，以尿酸作为碳/氮源制备的。该传感器基于内滤效应（IFE），其中秋水仙碱作为一种强大的吸收剂，影响荧光团（CQDs）的激发。这种重叠导致随着秋水仙碱浓度在 2-25 μM 范围内的增加，CQDs 的荧光定量衰减。所开发的传感器具有简单、耗时少、方便和令人满意的选择性等优点，可用于药物制剂中秋水仙碱的测定。
