Faculty of Science, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt.
Department of Zoology, Faulty of Science, Damanhour University, El Beheira, Egypt.
Sci Rep. 2022 May 19;12(1):8358. doi: 10.1038/s41598-022-12351-w.
Assessing the time of death based on the growth and development of insects is a critical task in forensic entomology. The rate of larvae development can be affected by a variety of toxins, including pesticides. Aluminum phosphide (AlP) is a low-cost insecticide that has yet to be tested for entomotoxicological significance, despite the fact that it is frequently the cause of fatal poisoning. In this study, we measured the body length of Chrysomya albiceps larvae reared on the carcasses of rabbits poisoned with AlP and analyzed the morphological changes of the larvae reared on the carcasses of rabbits poisoned with AlP. The concentration of AlP in the body of the larvae was significantly lower than in rabbit tissues. Insects from the AlP group had a significantly lower gain in body length. Furthermore, deformities in the larvae were found. Smaller respiratory spiracles were found, as well as a deformed small posterior end with hypogenesis of the posterior respiratory spiracles. Thus, disturbed growth and development of carrion flies found at a crime scene could indicate pesticide poisoning, such as aluminum phosphide.
基于昆虫的生长和发育来推断死亡时间是法医昆虫学中的一项关键任务。幼虫的发育速度可能会受到各种毒素的影响，包括杀虫剂。磷化铝（AlP）是一种低成本的杀虫剂，尽管它经常是致命中毒的原因，但尚未经过昆虫毒理学意义的测试。在这项研究中，我们测量了在被 AlP 中毒的家兔尸体上饲养的黄粉甲幼虫的体长，并分析了在被 AlP 中毒的家兔尸体上饲养的幼虫的形态变化。幼虫体内的 AlP 浓度明显低于兔组织中的浓度。来自 AlP 组的昆虫体长增长明显较低。此外，还发现了幼虫的畸形。发现较小的呼吸瓣，以及后呼吸瓣发育不良的小型后末端畸形。因此，在犯罪现场发现的腐食性蝇类生长发育受到干扰可能表明存在农药中毒，如磷化铝中毒。
