Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong, 637009, People's Republic of China.
Key Laboratories of Fine Chemicals and Surfactants in Sichuan Provincial Universities, Zigong, 643002, People's Republic of China.
Anal Bioanal Chem. 2022 Jul;414(16):4717-4726. doi: 10.1007/s00216-022-04094-4. Epub 2022 May 20.
A new fluorescent probe TPE-GHK was synthesized containing a tetrastyrene (TPE) derivative as fluorophore and classical tripeptide (Gly-His-Lys-NH) as a receptor based on the aggregation-induced emission (AIE) mechanism. TPE-GHK displayed high selectivity and rapid fluorescent "turn-on" response to Hg among other competitive metal ions. The 2:1 complex binding mechanism of TPE-GHK toward Hg was verified by fluorometric titration, Job's plots, and ESI-HRMS spectra. The fluorescent emission showed a good linear response in the range of 0-1.0 μM with the low detection limit of 28.6 nM. Meanwhile, TPE-GHK exhibited the excellent biocompatibility and low toxicity and was successfully applied in monitoring Hg in living CAKI 2 cells, which demonstrated its potential application in environment and biological science. More importantly, TPE-GHK could be used to detect Hg in two real water samples and also was successfully designed as test strips.
一种新型荧光探针 TPE-GHK 被合成，它包含一个四苯乙烯（TPE）衍生物作为荧光团和经典三肽（Gly-His-Lys-NH）作为受体，基于聚集诱导发射（AIE）机制。TPE-GHK 对 Hg 具有高选择性和快速荧光“开启”响应，而对其他竞争金属离子则没有响应。荧光滴定、Job 图和 ESI-HRMS 光谱验证了 TPE-GHK 与 Hg 的 2:1 络合结合机制。荧光发射在 0-1.0 μM 范围内呈现出良好的线性响应，检测限低至 28.6 nM。同时，TPE-GHK 表现出优异的生物相容性和低毒性，并成功应用于活 CAKI 2 细胞中 Hg 的监测，这证明了它在环境和生物科学中的潜在应用。更重要的是，TPE-GHK 可以用于检测两种实际水样中的 Hg，并且还成功设计为测试条。
