Department of Pathogen Biology and Immunology, Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Medical University, Xuzhou, China.
Department of Pathogen Biology, Key Laboratory of Tropical Disease Research of Guangdong Province, School of Public Health, Southern Medical University, Guangzhou, China.
Parasit Vectors. 2022 May 19;15(1):173. doi: 10.1186/s13071-022-05282-y.
Dengue is prevalent worldwide and is transmitted by Aedes mosquitoes. Temperature is a strong driver of dengue transmission. However, little is known about the underlying mechanisms.
Aedes albopictus mosquitoes exposed or not exposed to dengue virus serotype 2 (DENV-2) were reared at 23 °C, 28 °C and 32 °C, and midguts and residual tissues were evaluated at 7 days after infection. RNA sequencing of midgut pools from the control group, midgut breakthrough group and midgut nonbreakthrough group at different temperatures was performed. The transcriptomic profiles were analyzed using the R package, followed by weighted gene correlation network analysis (WGCNA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to identify the important molecular mechanisms regulated by temperature.
The midgut infection rate and midgut breakthrough rate at 28 °C and 32 °C were significantly higher than those at 23 °C, which indicates that high temperature facilitates DENV-2 breakthrough in the Ae. albopictus midgut. Transcriptome sequencing was performed to investigate the antiviral mechanism in the midgut. The midgut gene expression datasets clustered with respect to temperature, blood-feeding and midgut breakthrough. Over 1500 differentially expressed genes were identified by pairwise comparisons of midguts at different temperatures. To assess key molecules regulated by temperature, we used WGCNA, which identified 28 modules of coexpressed genes; the ME3 module correlated with temperature. KEGG analysis indicated that RNA degradation, Toll and immunodeficiency factor signaling and other pathways are regulated by temperature.
Temperature affects the infection and breakthrough of Ae. albopictus midguts invaded by DENV-2, and Ae. albopictus midgut transcriptomes change with temperature. The candidate genes and key pathways regulated by temperature provide targets for the prevention and control of dengue.
登革热在全球范围内流行，由埃及伊蚊传播。温度是登革热传播的主要驱动因素。然而，关于其潜在机制知之甚少。
将暴露于或未暴露于登革热病毒血清型 2（DENV-2）的白纹伊蚊饲养在 23°C、28°C 和 32°C 下，并在感染后 7 天评估中肠和残余组织。对不同温度下对照组、中肠突破组和中肠未突破组的中肠混合池进行 RNA 测序。使用 R 包分析转录组谱，然后进行加权基因相关网络分析（WGCNA）和京都基因与基因组百科全书（KEGG）分析，以确定受温度调节的重要分子机制。
28°C 和 32°C 时的中肠感染率和中肠突破率明显高于 23°C，表明高温有助于 DENV-2 在白纹伊蚊中肠突破。进行转录组测序以研究中肠的抗病毒机制。中肠基因表达数据集根据温度、吸血和中肠突破进行聚类。通过比较不同温度下的中肠，鉴定出 1500 多个差异表达基因。为了评估受温度调节的关键分子，我们使用 WGCNA 鉴定了 28 个共表达基因模块；ME3 模块与温度相关。KEGG 分析表明，RNA 降解、Toll 和免疫缺陷因子信号等途径受温度调节。
温度影响 DENV-2 感染和突破白纹伊蚊中肠，白纹伊蚊中肠转录组随温度变化。候选基因和受温度调节的关键途径为登革热的预防和控制提供了目标。
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