Fu Wenqi, Liu Shuang, Jiao Jun, Xie Zhiwen, Huang Xinfang, Lu Yun, Liu Huiying, Hu Shuhai, Zuo Enjun, Kou Ni, Ma Guowu
Department of Oral Prosthodontics, School of Stomatology, Dalian Medical University, Lvshun South Road, Dalian 116044, China.
Academician Laboratory of Immune and Oral Development & Regeneration, Dalian Medical University, Lvshun South Road, Dalian 116044, China.
Materials (Basel). 2022 May 2;15(9):3263. doi: 10.3390/ma15093263.
Cobalt-chromium (Co-Cr) alloys have been widely used as dental-restoration materials for many years. This study sought to investigate whether selective laser melting (SLM) is a more appropriate process than traditional casting (CAST) for fabricating dental Co-Cr alloys. Metallurgical microscopy, X-ray photoelectron spectroscopy (XPS), Vickers hardness and nanoindentation tests, and friction and wear tests were used to evaluate the microstructure, surface compositions, mechanical properties, and wear resistance, respectively. Additionally, the biocompatibilities and cell adhesion of the alloys were evaluated with L-929 fibroblasts via CCK-8 assay, Live/Dead staining, flow cytometric analysis, scanning electron microscopy (SEM) observation and real-time PCR (RT-PCR) assay. The XPS results showed that the two alloys were all mainly comprised of Co, Cr, and O. The hardness in the CAST group equaled 7.15 ± 0.48 GPa, while in the SLM group, it equaled 9.06 ± 0.49 GPa. The friction coefficient of SLM alloys remained at approximately 0.46, but the CAST specimens fluctuated significantly. SLM alloys exhibited shallower wear scars and less wear debris compared with CAST alloys, simultaneously. Additionally, there were higher survival and expression of cell-adhesion-related genes on SLM alloys of L-929 cells, which meant that the deleterious effect on L-929 cells was significantly reduced compared with that for the CAST alloys. Overall, the wear resistances and biocompatibilities of the Co-Cr dental alloys were dramatically affected by the fabrication technique. The SLM technique is advantageous over the CAST technique for fabricating Co-Cr dental alloys.
钴铬(Co-Cr)合金多年来一直被广泛用作牙科修复材料。本研究旨在探究选择性激光熔化(SLM)是否比传统铸造(CAST)更适合用于制造牙科Co-Cr合金。分别使用金相显微镜、X射线光电子能谱(XPS)、维氏硬度和纳米压痕测试以及摩擦磨损测试来评估微观结构、表面成分、力学性能和耐磨性。此外,通过CCK-8测定、活/死染色、流式细胞术分析、扫描电子显微镜(SEM)观察和实时聚合酶链反应(RT-PCR)测定,用L-929成纤维细胞评估了合金的生物相容性和细胞粘附性。XPS结果表明,两种合金均主要由Co、Cr和O组成。CAST组的硬度为7.15±0.48 GPa,而SLM组的硬度为9.06±0.49 GPa。SLM合金的摩擦系数保持在约0.46,但CAST试样波动明显。同时,与CAST合金相比,SLM合金的磨损痕迹更浅,磨损碎屑更少。此外,L-929细胞在SLM合金上的细胞粘附相关基因的存活率和表达更高,这意味着与CAST合金相比,对L-929细胞的有害影响显著降低。总体而言,Co-Cr牙科合金的耐磨性和生物相容性受制造技术的显著影响。在制造Co-Cr牙科合金方面,SLM技术优于CAST技术。