Fujii T, Maeda M, Kawashima Y
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Mar;39(3):352-8.
Hitherto, the selection of anticancer drug has been based on clinical experience. But to succeed for chemotherapy to succeed, effective drugs must be selected for individual patients with cancer. We have developed the following new in vitro micromethod. Method. In brief, tumor cells obtained by surgery were incubated with anticancer drugs for 48 hrs at 37 degrees C in 5% CO2 in a microplate. After incubation, the surviving cells were fixed with methanol and stained with crystal violet. Then the stained cells were solubilized with 1% lauryl sulfate and the absorbance of each well was measured at 540 nm with a multiscan spectrophotometer. In this method, cytotoxicity was quantitated by the absorbance. The method is simple, rapid (48 hrs) and reproducible, and it requires only a small amount of cells (5 X 10(3) approximately 1 X 10(4]. This method correlates well with sensitivity tests using isotopes. We have examined the chemosensitivity of 22 specimens from gynecologic malignancies by this method. The success rate is 77% and higher than with any other in vitro method. This method will be widely utilized in several fields of cancer treatment in the near future.
迄今为止，抗癌药物的选择一直基于临床经验。但是为了使化疗取得成功，必须为个体癌症患者选择有效的药物。我们开发了以下新的体外微量方法。方法。简而言之，将手术获取的肿瘤细胞与抗癌药物在微孔板中于37℃、5%二氧化碳条件下孵育48小时。孵育后，用甲醇固定存活细胞，并用结晶紫染色。然后用1%十二烷基硫酸钠溶解染色细胞，用多扫描分光光度计在540nm处测量各孔的吸光度。在该方法中，细胞毒性通过吸光度进行定量。该方法简单、快速（48小时）且可重复，并且只需要少量细胞（5×10³至1×10⁴）。该方法与使用同位素的敏感性试验相关性良好。我们用该方法检测了22例妇科恶性肿瘤标本的化疗敏感性。成功率为77%，高于任何其他体外方法。该方法在不久的将来将在癌症治疗的多个领域得到广泛应用。
