State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin, China.
College of Life Sciences, Fujian Normal University, Fuzhou, China.
J Food Sci. 2022 Jun;87(6):2463-2473. doi: 10.1111/1750-3841.16167. Epub 2022 May 20.
This research aims to prepare capsules emulsion using gallic acid (GA), dextran (DEX), bovine serum albumin (BSA), sodium alginate, and K-carrageenan (K-Car) as the biological delivery system of lycopene. The stability and bioaccessibility of lycopene were further improved through encapsulation of covalent complex of sodium alginate and K-Car. The molecular weight distribution and secondary structure of the conjugates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared spectroscopy (FTIR). The storage stability of the emulsion stabilized by conjugates was measured with Turbiscan stability index (TSI) and fluctuation of the particle size. The TSI value of ternary conjugates was 18.7 (37℃) with particle sizes ranging from 208 to 319 nm. Then, the changes of three-dimensional reticulate structures and physical properties of sodium alginate-K were analyzed by scanning electron microscopy (SEM) and TPA. The thermal stability of the sodium alginate-K-Car composite systems was increased compared with sodium alginate. The bioaccessibility of lycopene was significantly improved under the dual embedding of BSA-DEX-GA conjugate emulsion and sodium alginate-K-Car composite systems.
本研究旨在制备使用没食子酸（GA）、葡聚糖（DEX）、牛血清白蛋白（BSA）、海藻酸钠和κ-卡拉胶（K-Car）作为番茄红素生物传递系统的胶囊乳液。通过包埋海藻酸钠和 K-Car 的共价复合物，进一步提高了番茄红素的稳定性和生物利用度。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和傅里叶变换红外光谱（FTIR）分析了共轭物的分子量分布和二级结构。用 Turbiscan 稳定性指数（TSI）和粒径波动测量了由共轭物稳定的乳液的储存稳定性。三元共轭物的 TSI 值为 18.7（37℃），粒径范围为 208 至 319nm。然后，通过扫描电子显微镜（SEM）和 TPA 分析了海藻酸钠-K 的三维网状结构和物理性质的变化。与海藻酸钠相比，海藻酸钠-K-Car 复合体系的热稳定性得到了提高。在 BSA-DEX-GA 共轭乳液和海藻酸钠-K-Car 复合体系的双重包埋下，番茄红素的生物利用度显著提高。
