Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic‑induced external apical root resorption in a rat model.

The aim of the present study was to investigate the alterations in the formation of cementocytes in response to orthodontic forces and to evaluate the contribution of these cells in the biological changes of tooth movement and associated root resorption. A total of 90 Sprague Dawley rats were randomly assigned to the control, high force, and low force groups. Intrusion forces of 10 and 50 g were applied on the rat molar to induce tooth intrusion. The tooth movement was observed from 0 to 14 days by micro‑computed tomography, bone histometric analysis, tartrate‑resistant acid phosphatase staining, as well as reverse transcription‑quantitative PCR and immunofluorescence staining assays. The results suggested that under low force conditions, osteoclasts were distributed at a higher frequency on the bone side than on the root side. Under high force conditions, both sides suffered osteoclast infiltration. In the low force group, the cementocytes exhibited downregulated sclerostin () and osteoprotegerin () mRNA levels and a lower receptor activator of nuclear factor‑κB ligand ()/OPG ratio over a certain period of time. The expression levels of these genes were lower compared with those of the osteocytes at each time‑point. In the high force group, both cementocytes and osteocytes upregulated the and ratio on days 7 and 14, while the cementocytes expressed higher levels of mRNA than those noted in the osteocytes. These data suggested that cementocytes responded to the orthodontic force via modulation of the ratio and expression. The biological response of cementocytes contributed to the mechanotransduction and homoeostasis of the roots under compression. Excessive forces may act as a negative factor of this regulatory role. These results expand our knowledge on the function of cementocytes.