来自猪嗅觉黏膜的细胞悬液。对各种气味剂的膜电位和膜流动性变化。
Cell suspensions from porcine olfactory mucosa. Changes in membrane potential and membrane fluidity in response to various odorants.
作者信息
Kashiwayanagi M, Sai K, Kurihara K
出版信息
J Gen Physiol. 1987 Mar;89(3):443-57. doi: 10.1085/jgp.89.3.443.
A suspension of olfactory epithelial cells was prepared from porcine olfactory mucosa and the physiological functions of the suspension were examined. The membrane potential of the cell suspension, which was monitored by measuring the fluorescence changes of rhodamine 6G, was depolarized by an increase in the K+ concentration in the external medium. Various odorants depolarized the cell suspension in a dose-dependent fashion. The magnitude of depolarization by odorants was either unchanged or slightly increased by a reduction of the concentration of Na+, Ca2+, and Cl- in the external medium, which suggests that changes in the permeabilities of specific ions are not involved in depolarization by odorants. The application of various odorants to the cell suspension induced changes in the membrane fluidity at different sites of the membrane that were monitored with various fluorescent dyes [8-anilino-1-naphthalene sulfonate, n-(9-anthroyloxy) stearic acids, 12-(9-anthroyloxy) oleic acid, and (1,6-diphenyl-1,3,5-hexatriene)], which suggests that the odorants having different odors are adsorbed on different sites in the membrane. On the basis of these results, a possible mechanism of odor discrimination is discussed.
从猪嗅觉黏膜制备嗅觉上皮细胞悬液，并检测该悬液的生理功能。通过测量罗丹明6G的荧光变化来监测细胞悬液的膜电位，外部介质中K⁺浓度的增加使膜电位去极化。各种气味剂以剂量依赖的方式使细胞悬液去极化。外部介质中Na⁺、Ca²⁺和Cl⁻浓度降低时，气味剂引起的去极化幅度要么不变，要么略有增加，这表明特定离子通透性的变化与气味剂引起的去极化无关。用各种荧光染料[8-苯胺基-1-萘磺酸、n-(9-蒽氧基)硬脂酸、12-(9-蒽氧基)油酸和(1,6-二苯基-1,3,5-己三烯)]监测，将各种气味剂施加到细胞悬液中会引起膜不同部位的膜流动性变化，这表明具有不同气味的气味剂吸附在膜的不同部位。基于这些结果，讨论了气味辨别可能的机制。
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