Nilsson G E, Tottmar O
J Neurochem. 1987 May;48(5):1566-72. doi: 10.1111/j.1471-4159.1987.tb05702.x.
When 1 mM serotonin, dopamine, or norepinephrine was incubated with a monoamine oxidase preparation (mitochondrial membranes) in the presence of 4 mM sodium bisulfite, 85-95% of the amines were oxidized to the corresponding aldehydes. In the absence of bisulfite, the recoveries were only approximately 30%, and dark colored products were formed during the incubations. The aldehydes derived from tyramine, octopamine, methoxytyramine, and normetanephrine were also prepared by the use of this method. The bisulfite-aldehyde compounds were stable during storage at -20 degrees C. Bisulfite-free aldehyde solutions were made by diethylether extraction. When the aldehydes derived from dopamine or serotonin were incubated with rat brain homogenates, they were found to disappear in an aldehyde dehydrogenase- and aldehyde reductase-independent manner. The disappearance of the latter aldehyde was more pronounced, and the results indicated that this aldehyde may react with both proteins and phospholipids.
当1 mM的血清素、多巴胺或去甲肾上腺素与单胺氧化酶制剂（线粒体膜）在4 mM亚硫酸氢钠存在的情况下一起孵育时，85 - 95%的胺被氧化为相应的醛。在没有亚硫酸氢盐的情况下，回收率仅约为30%，并且在孵育过程中会形成深色产物。通过使用这种方法还制备了来自酪胺、章鱼胺、甲氧基酪胺和去甲变肾上腺素的醛。亚硫酸氢盐 - 醛化合物在 - 20℃储存期间是稳定的。无亚硫酸氢盐的醛溶液通过乙醚萃取制备。当将来自多巴胺或血清素的醛与大鼠脑匀浆一起孵育时，发现它们以不依赖醛脱氢酶和醛还原酶的方式消失。后一种醛的消失更为明显，结果表明这种醛可能与蛋白质和磷脂都发生反应。
