INFN Firenze, via Sansone 1, 50019, Sesto Fiorentino, Italy.
ISIS "Leonardo da Vinci", via del Terzolle 91, 50127, Florence, Italy.
Eur Biophys J. 2022 Jul;51(4-5):413-418. doi: 10.1007/s00249-022-01603-2. Epub 2022 May 23.
The elastic properties of the double-stranded DNA handles used in optical tweezers experiments on biomolecules are customarily modeled by an extensible worm-like chain model. Fitting such a model to experimental data, however, is no trivial task, as the function depends on four parameters in a highly non-linear fashion. We hereby propose a method to bypass the fitting procedure and obtain an empirical force vs. extension curve that accurately reproduces the elasticity of the handles.
在生物分子的光学镊子实验中，双链 DNA 手柄的弹性特性通常通过可伸缩的蠕虫链模型来模拟。然而，拟合这样的模型并不是一件简单的事情，因为该函数以高度非线性的方式取决于四个参数。在此，我们提出了一种绕过拟合过程的方法，得到了一个经验力与伸长曲线，该曲线可以准确地再现手柄的弹性。
