Gou Yu-Ping, Quandahor Peter, Mao Liang, Li Chun-Chun, Zhou Jing-Jiang, Liu Chang-Zhong
College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, China.
CSIR-Savanna Agricultural Research Institute, Tamale, Ghana.
Front Physiol. 2022 May 5;13:879401. doi: 10.3389/fphys.2022.879401. eCollection 2022.
Botanical pesticide is highly recommended for integrated pest management (IPM), due to its merits such as environmental friendliness, safe to non-target organisms, operators, animals, and food consumers. The experiment was conducted to determine the lethal and sub-lethal effects of allyl isothiocyanate (AITC) on eggs, third instar larvae, pupae, and females and males of Johannsen (). Different concentrations of AITC under ambient CO by the conical flask sealed fumigation method were used for the experiment. The results showed that there was a significant linear relationship between different concentrations of AITC and the toxicity regression equation of . The sub-lethal concentrations of AITC had significant effects on the larval stage, pupal stage, pupation rate, pupal weight, adult emergence rate, and oviposition. The pupation rate, pupal weight, and adult emergency rate were significantly ( < 0.05) affected by AITC fumigation. The pupation rate was the lowest after fumigation treatment of AITC at LC (36.67%), followed by LC (41.94%), compared with the CK (81.39%). Female longevity was significantly ( < 0.05) shortened by fumigation at LC (1.75 d) and LC (1.64 d), compared with that of CK (2.94 d). Male longevity was shorter at LC (1.56 d) than at LC (1.25 d) and had no significant difference between these two treatments. The fumigation efficiency of AITC was significantly increased under high CO condition. Furthermore, detoxification enzyme activities and antioxidant enzyme activities were accumulated under high CO condition. The fumigation method in the application of AITC can be useful in areas where is a major concern.
植物源农药因其具有环境友好、对非靶标生物、操作人员、动物和食品消费者安全等优点，在害虫综合治理（IPM）中备受推荐。本实验旨在测定异硫氰酸烯丙酯（AITC）对Johannsen的卵、三龄幼虫、蛹以及雌成虫和雄成虫的致死和亚致死效应。采用锥形瓶密封熏蒸法，在环境CO₂条件下使用不同浓度的AITC进行实验。结果表明，不同浓度的AITC与毒性回归方程之间存在显著的线性关系。AITC的亚致死浓度对幼虫期、蛹期、化蛹率、蛹重、成虫羽化率和产卵有显著影响。AITC熏蒸显著（P<0.05）影响化蛹率、蛹重和成虫羽化率。与对照（CK，81.39%）相比，AITC在LC₅₀熏蒸处理后的化蛹率最低（36.67%），其次是LC₁₀（41.94%）。与CK（2.94天）相比，LC₅₀（1.75天）和LC₁₀（1.64天）熏蒸显著（P<0.05）缩短了雌成虫寿命。雄成虫在LC₅₀（1.56天）的寿命短于LC₁₀（1.25天），且这两种处理之间无显著差异。在高CO₂条件下，AITC的熏蒸效率显著提高。此外，在高CO₂条件下解毒酶活性和抗氧化酶活性增加。AITC应用中的熏蒸方法在[具体问题]是主要关注点的地区可能会很有用。
