Department of Pesticide Science, College of Plant Protection, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
Key Laboratory for Agro-biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
BMC Microbiol. 2023 Sep 30;23(1):278. doi: 10.1186/s12866-023-02992-w.
Allyl isothiocyanate (AITC) is a natural product with high volatility that is used as a biofumigant to alleviate soil-borne plant diseases, and problems such as root knot nematodes (RKNs) that necessitate continuous cropping. However, little research has assessed the effects of AITC fumigation on medicinal plants.
AITC significantly reduced the population of RKNs in soil (p < 0.0001) and showed an excellent RKN disease control effect within 6 months after sowing Panax notoginseng (p < 0.0001). The seedling survival rate of 2-year-old P. notoginseng was approximately 1.7-fold higher after soil treatment with AITC (p = 0.1008). 16S rRNA sequencing indicated that the AITC treatment affected bacterial richness rather than diversity in consecutively cultivated (CC) soil. Furthermore, biomarkers with statistical differences between AITC-treated and untreated CC soil showed that Pirellulales (order), Pirellulaceae (family), Pseudomonadaceae (family), and Pseudomonas (genus) played important roles in the AITC-treated group. In addition, the microbiome functional phenotypes predicted using the BugBase tool suggested that AITC treatment is more conducive to improving CC soil through changes in the bacterial community structure. Crucially, our research also suggested that AITC soil treatment significantly increases soil organic matter (p = 0.0055), total nitrogen (p = 0.0054), and available potassium (p = 0.0373), which promotes the survival of a succeeding medicinal plant (Polygonatum kingianum).
AITC is an ecologically friendly soil treatment that affects the top 10 bacterial richness but not diversity. It could also provide a basis for a useful agricultural soil management measure to alleviate soil sickness.
丙烯基异硫氰酸酯（AITC）是一种挥发性高的天然产物，用作生物熏蒸剂来缓解土传植物病害和连作问题，如根结线虫（RKN）。然而，很少有研究评估 AITC 熏蒸对药用植物的影响。
AITC 显著降低了土壤中 RKN 的种群（p<0.0001），并在播种人参（Panax notoginseng）后 6 个月内表现出极好的 RKN 病害防治效果（p<0.0001）。经过 AITC 土壤处理后，2 年生人参的幼苗成活率提高了约 1.7 倍（p=0.1008）。16S rRNA 测序表明，AITC 处理影响了连续种植（CC）土壤中的细菌丰富度，而不是多样性。此外，在 AITC 处理和未处理的 CC 土壤之间具有统计学差异的生物标志物表明，节杆菌目（order）、节杆菌科（family）、假单胞菌科（family）和假单胞菌属（genus）在 AITC 处理组中发挥了重要作用。此外，使用 BugBase 工具预测的微生物组功能表型表明，AITC 处理更有利于通过改变细菌群落结构来改善 CC 土壤。至关重要的是，我们的研究还表明，AITC 土壤处理显著增加了土壤有机质（p=0.0055）、总氮（p=0.0054）和有效钾（p=0.0373），这促进了后续药用植物（黄精）的存活。
AITC 是一种生态友好型土壤处理方法，它影响前 10 位的细菌丰富度但不影响多样性。它还可以为缓解土壤疾病提供一种有用的农业土壤管理措施的基础。
