Institut de Génétique Humaine, CNRS, Université de Montpellier, Molecular Basis of Inflammation Laboratory, 34090 Montpellier, France.
STAR Protoc. 2022 May 14;3(2):101384. doi: 10.1016/j.xpro.2022.101384. eCollection 2022 Jun 17.
The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway plays a pivotal role in several cellular processes including pathogen recognition and inflammatory responses. We describe a protocol to activate the cGAS-STING pathway in murine cells using nucleic acids transfection. We describe how to prepare the nucleic acid probes and validate activation of the pathway by western blot and gene expression analysis. The protocol can be applied to investigate cGAS-STING signaling in both murine and human cell lines. For complete details on the use and execution of this protocol, please refer to Vila et al. (2022)
环鸟苷酸-腺苷酸合成酶 (cGAS)-干扰素基因刺激物 (STING) 途径在包括病原体识别和炎症反应在内的几种细胞过程中发挥着关键作用。我们描述了一种使用核酸转染在鼠细胞中激活 cGAS-STING 途径的方案。我们描述了如何制备核酸探针，并通过 Western blot 和基因表达分析验证途径的激活。该方案可用于研究鼠和人细胞系中的 cGAS-STING 信号。有关此方案的使用和执行的完整详细信息，请参阅 Vila 等人。（2022 年）
