College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China.
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China.
J Proteomics. 2019 Sep 30;208:103470. doi: 10.1016/j.jprot.2019.103470. Epub 2019 Jul 30.
Lonicera japonica Thunb. is an important medicinal plant. The secondary metabolites in L. japonica are diverse and vary in levels during development, leading to the ambiguous evaluation for its medical value. In order to reveal the regulatory mechanism of secondary metabolites during the flowering stages, transcriptomic, proteomic, and metabolomic analyses were performed. The integration analysis of omic-data illustrated that the metabolic changes over the flower developmental stages were mainly involved in sugar metabolism, lipopolysaccharide biosynthesis, carbon conversion, and secondary metabolism. Further proteomic analysis revealed that uniquely identified proteins were mainly involved in glycolysis/phenylpropanoids and tricarboxylic acid cycle/terpenoid backbone pathways in early and late stages, respectively. Transketolase was commonly identified in the 5 developmental stages and 2-fold increase in gold flowering stage compared with juvenile bud stage. Simple phenylpropanoids/flavonoids and 1-deoxy-D-xylulose-5-phosphate were accumulated in early stages and upregulated in late stages, respectively. These results indicate that phenylpropanoids were accumulated attributing to the activated glycolysis process in the early stages, while the terpenoids biosynthetic pathways might be promoted by the transketolase-contained regulatory circuit in the late stages of L. japonica flower development. BIOLOGICAL SIGNIFICANCE: Lonicera japonica Thunb. is a native species in the East Asian and used in traditional Chinese medicine. In order to reveal the regulatory mechanism of secondary metabolites during the flowering stages, transcriptomic, proteomic, and metabolomic analyses were performed. The integration analysis of omic-data illustrated that the metabolic changes over the flower developmental stages were mainly involved in sugar metabolism, lipopolysaccharide biosynthesis, carbon conversion, and secondary metabolism. Our results indicate that phenylpropanoids were accumulated attributing to the activated glycolysis process in the early stages, while the terpenoids biosynthetic pathways might be promoted by the transketolase-contained regulatory circuit in the late stages of L. japonica flower development.
忍冬是一种重要的药用植物。忍冬中的次生代谢产物种类多样，在发育过程中含量也不同，这导致其药用价值的评价存在一定的模糊性。为了揭示其开花阶段次生代谢物的调控机制，我们进行了转录组、蛋白质组和代谢组学分析。对组学数据的综合分析表明，花发育阶段的代谢变化主要涉及糖代谢、脂多糖生物合成、碳转化和次生代谢。进一步的蛋白质组分析表明，在早期和晚期阶段分别有独特鉴定的蛋白质主要参与糖酵解/苯丙素和三羧酸循环/萜烯骨架途径。转酮醇酶在 5 个发育阶段和金花期均有鉴定，且与幼芽期相比，金花期表达量增加了 2 倍。简单苯丙素/类黄酮和 1-脱氧-D-木酮糖-5-磷酸在早期积累，并在晚期上调。这些结果表明，苯丙素的积累归因于早期糖酵解过程的激活，而萜烯类生物合成途径可能是由晚期转酮醇酶调控回路所促进。
忍冬是东亚的本地种，用于传统中药。为了揭示其开花阶段次生代谢物的调控机制，我们进行了转录组、蛋白质组和代谢组学分析。对组学数据的综合分析表明，花发育阶段的代谢变化主要涉及糖代谢、脂多糖生物合成、碳转化和次生代谢。我们的结果表明，苯丙素的积累归因于早期糖酵解过程的激活，而萜烯类生物合成途径可能是由晚期转酮醇酶调控回路所促进。
