Ersoy Tuğba, Ozmen Ozlem
Vet Res Forum. 2022 Mar;13(1):127-131. doi: 10.30466/vrf.2020.116534.2772. Epub 2022 Mar 15.
Department of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkiye. Canine parvovirus (CPV) causes a contagious and fatal viral disease in dogs characterized by hemorrhagic enteritis. Apoptosis is a programmed cell death and one of the primary markers of this process is caspase 3. Proliferating cell nuclear antigen (PCNA) is also associated with important vital cellular processes. This study was conducted to examine the expressions of caspase 3 and PCNA in the intestinal samples of dogs naturally infected with CPV using immunohistochemical methods. A total of 30 dogs with parvoviral enteritis and five control dogs gut tissues were evaluated for caspase 3 and PCNA expressions. Increased immunoactivities of caspase 3 and PCNA were observed in epithelial, crypt and inflammatory cells in the CPV-infected dogs. Increased expressions of both markers were observed being related to the severity of disease. These results demonstrated the important roles of caspase 3 and PCNA in CPV pathogenesis. These markers may be useful for early diagnosis, estimation of the severity or future treatment strategies of this important disease.
土耳其布尔杜尔市布尔杜尔梅赫梅特·阿基夫·埃尔索伊大学兽医学院病理学系。犬细小病毒（CPV）在犬类中引发一种具有传染性的致命病毒性疾病，其特征为出血性肠炎。细胞凋亡是一种程序性细胞死亡，该过程的主要标志物之一是半胱天冬酶3。增殖细胞核抗原（PCNA）也与重要的细胞生命过程相关。本研究旨在采用免疫组织化学方法检测自然感染CPV的犬肠道样本中半胱天冬酶3和PCNA的表达情况。共对30只患有细小病毒性肠炎的犬以及5只对照犬的肠道组织进行了半胱天冬酶3和PCNA表达情况的评估。在感染CPV的犬的上皮细胞、隐窝细胞和炎性细胞中观察到半胱天冬酶3和PCNA的免疫活性增加。观察到这两种标志物的表达增加与疾病严重程度相关。这些结果证明了半胱天冬酶3和PCNA在CPV发病机制中的重要作用。这些标志物可能有助于这种重要疾病的早期诊断、病情严重程度评估或未来治疗策略的制定。
