用于原位表面增强拉曼散射检测和靶向化学-光动力协同治疗的DNA-金纳米 Janus 粒子
DNA-Au Janus Nanoparticles for In Situ SERS Detection and Targeted Chemo-photodynamic Synergistic Therapy.
作者信息
Zhang Xiaoru, Ge Yonghao, Liu Minghui, Pei Yujiao, He Peng, Song Weiling, Zhang Shusheng
机构信息
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, and College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China.
College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China.
出版信息
Anal Chem. 2022 May 22. doi: 10.1021/acs.analchem.1c05649.
Cancer theranostics is of great significance in the personalized therapy. In this work, stable Janus nanoparticles (JNPs) containing PEG and two kinds of DNAs were prepared by means of "click chemistry". In response to ATP or acid condition, the prepared JNPs could form Au NP dimers, which facilitate in situ SERS detection and SERS imaging analysis of cancer cells due to the formation of "hot spots" in the nanogap between the Au NP dimers. A detection limit of 2.3 × 10 M was obtained for ATP. As for a pH sensor, the SERS signals increased with the decrease of pH value from 8.0 to 4.0. In situ monitoring of ATP or acid condition in cancer cells by SERS can improve the accuracy and sensitivity of diagnosis. Moreover, drugs and photosensitizers loaded on the other side of JNPs led to the chemotherapy/photodynamic therapy synergistic antitumor effect, which was verified by in vitro and in vivo experiments. Given the excellent performance in SERS detection and cancer therapy, the developed JNPs hold considerable potential in cancer theranostics.
癌症诊疗一体化在个性化治疗中具有重要意义。在本研究中，通过“点击化学”方法制备了含有聚乙二醇（PEG）和两种DNA的稳定Janus纳米颗粒（JNPs）。响应ATP或酸性条件时，所制备的JNPs可形成金纳米颗粒二聚体，由于金纳米颗粒二聚体之间纳米间隙中形成“热点”，这有助于癌细胞的原位表面增强拉曼光谱（SERS）检测和SERS成像分析。ATP的检测限为2.3×10⁻⁸ M。作为pH传感器，当pH值从8.0降至4.0时，SERS信号增强。通过SERS对癌细胞内ATP或酸性条件进行原位监测可提高诊断的准确性和灵敏度。此外，负载在JNPs另一侧的药物和光敏剂可产生化疗/光动力治疗协同抗肿瘤效果，这已通过体外和体内实验得到验证。鉴于其在SERS检测和癌症治疗方面的优异性能，所开发的JNPs在癌症诊疗一体化中具有巨大潜力。
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