Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.
Project Research Center for Fundamental Sciences, Graduate Scholl of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan.
Chemistry. 2022 Jul 1;28(37):e202201253. doi: 10.1002/chem.202201253. Epub 2022 May 23.
Glycosylation of proteins is known to be essential for changing biological activity and stability of glycoproteins on the cell surfaces and in body fluids. Delivering of homogeneous glycoproteins into the endoplasmic reticulum (ER) and the Golgi apparatus would enable us to investigate the function of asparagine-linked (N-) glycans in the organelles. In this work, we designed and synthesized an intentionally glycosylated cholera toxin B-subunit (CTB) to be transported to the organelles of mammalian cells. The heptasaccharide, the intermediate structure of various complex-type N-glycans, was introduced to the CTB. The synthesized monomeric glycosyl-CTB successfully entered mammalian cells and was transported to the Golgi and the ER, suggesting the potential use of synthetic CTB to deliver and investigate the functions of homogeneous N-glycans in specific organelles of living cells.
糖基化蛋白质对于改变细胞表面和体液中糖蛋白的生物活性和稳定性是至关重要的。将同质糖蛋白递送到内质网（ER）和高尔基体中，使我们能够研究天冬酰胺连接（N-）聚糖在细胞器中的功能。在这项工作中，我们设计并合成了一种有意糖基化的霍乱毒素 B 亚基（CTB），以将其运输到哺乳动物细胞的细胞器中。引入了七糖，即各种复杂型 N-聚糖的中间结构，到 CTB 中。合成的单糖基 CTB 成功进入哺乳动物细胞，并被运输到高尔基体和 ER，这表明合成 CTB 有可能用于递送到活细胞的特定细胞器中，并研究同质 N-聚糖的功能。
