Cellular and Molecular Research Center, Medical Basic Sciencec Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
Turkiye Parazitol Derg. 2022 May 23;46(2):91-96. doi: 10.4274/tpd.galenos.2021.53825.
Recently, the use of pentavalent antimony compounds for treatment has been associated with disease recurrence, drug resistance, and severe side effects. Therefore, there is a need to develop alternative treatment strategies. This study investigates the effects of on promastigotes and amastigotes of and .
Promastigotes and amastigotes of and were cultured and mass-produced in an RPMI1640 medium enriched with other necessary compounds. The MTT colorimetric method and calculating the IC50 value were used to evaluate the anti-leishmania activity of hydroalcoholic extract of .
The hydroalcoholic extract of inhibited the growth of and promastigotes in 24, 48, and 72 hours after incubation. The IC50 of hydroalcoholic extract of was 56 μg/mL for and 275 μg/mL for promastigotes after 72 hours. The IC50 of hydroalcoholic extract of was 75 μg/mL for and 325 μg/mL for amastigotes after 72 hours.
The results showed that hydroalcoholic extract of has cytotoxicity properties, and has a higher resistance to hydroalcoholic extract of than . Further research is recommended.
最近，五价锑化合物的使用与疾病复发、耐药性和严重的副作用有关。因此，需要开发替代治疗策略。本研究调查了 对 株和 株的影响。
在富含其他必需化合物的 RPMI1640 培养基中培养和大量生产 和 的前鞭毛体和无鞭毛体。使用 MTT 比色法和计算 IC50 值来评估 水醇提取物对利什曼原虫的抗活性。
水醇提取物抑制 株和 株在孵育 24、48 和 72 小时后的生长。水醇提取物的 IC50 为 56μg/mL ，72 小时后为 275μg/mL 。水醇提取物的 IC50 为 75μg/mL ，72 小时后为 325μg/mL 。
结果表明，水醇提取物具有细胞毒性，而 对水醇提取物的抗性高于 。建议进一步研究。
