Erciyes Üniversitesi Tıp Fakültesi, Parazitoloji Anabilim Dalı, Kayseri, Türkiye
Turkiye Parazitol Derg. 2022 May 23;46(2):133-139. doi: 10.4274/tpd.galenos.2022.32032.
Cystic echinococcosis (CE) is a parasitic disease that has been known for years in helminth diseases and it is important as human and animal health problem in many parts of the world and in our country due to economic losses. In this study, it was aimed to retrospectively evaluate the distribution of anti--IgG antibodies in patients with pre-diagnosis of CE that referred to parasitology laboratory between January 2013-December 2018.
Commercial kit was used for indirect hemaglutination (IHA), indirect fluorescent antibody test (IFAT) and Western blot (WB) methods using sera from patient samples was applied according to the kit proposal. In addition, patient materials for CAM, CSF and blood for which polymerase chain reaction (PCR)/QPCR tests were requested were examined.
Sera of the patients who were tested with at least one of the IHA, IFAT and WB methods or a combination of these methods, and 443 cases out of 2.283 cases were found to be seropositive. It was determined that 369 (62.03%) of 443 positive patients were female and 330 (37.97%) were male patients. Among these patients, 87 patients whose IFAT and/or IHA tests were negative were found to have positive results with the WB method. IFAT or IHA test results of 13 patients with negative WB tests were found to be positive. Four patients were identified with both tests positive but WB test results negative. In addition, 36 of 72 patients who underwent PCR/QPCR tests were found to be positive.
As a result of a six-year retrospective screening, 22% of the cases were found to be positive, and it was concluded that the prevalence of CE is high and the use of a single test may be insufficient in the diagnosis of CE, therefore, test combinations will increase the sensitivity and reliability in reaching the correct diagnosis.
囊型包虫病（CE）是一种寄生虫病，在多年的蠕虫病研究中已被认识，并且由于经济损失，在世界许多地区和我国，它是一个重要的人类和动物健康问题。在这项研究中，我们旨在回顾性评估 2013 年 1 月至 2018 年 12 月间寄生虫学实验室就诊的疑似 CE 患者的抗 IgG 抗体分布情况。
使用间接血凝法（IHA）、间接荧光抗体试验（IFAT）和 Western blot（WB）试剂盒，根据试剂盒说明书，对患者血清进行检测。此外，还对要求进行聚合酶链反应（PCR）/QPCR 检测的 CAM、CSF 和血液患者材料进行了检查。
对至少使用 IHA、IFAT 和 WB 方法之一或这些方法组合进行检测的患者血清进行检测，在 2283 例患者中，443 例血清呈阳性。确定 443 例阳性患者中，369 例（62.03%）为女性，330 例（37.97%）为男性。在这些患者中，87 例 IFAT 和/或 IHA 检测阴性的患者，WB 方法检测呈阳性。13 例 WB 检测阴性的 IFAT 或 IHA 检测结果为阳性。4 例患者两种检测均为阳性，但 WB 检测结果为阴性。此外，在 72 例接受 PCR/QPCR 检测的患者中，有 36 例检测结果为阳性。
六年回顾性筛查结果显示，22%的患者呈阳性，表明 CE 的患病率较高，单一检测可能不足以诊断 CE，因此，联合检测将提高诊断的敏感性和可靠性。
