Kobayashi S, Takai K, Masuda S, Inoue A
Jpn J Antibiot. 1986 Dec;39(12):3148-55.
Combination therapy of aminoglycoside and beta-lactam antibiotics is often used clinically because of its effectiveness. This paper reports a high performance liquid chromatographic (HPLC) determination method for each of astromicin (ASTM) and cefoperazone (CPZ) in blood samples of several species. The procedure for ASTM included purification with CM-Sephadex, separation on a reversed phase column (C-18) and derivatization with o-phthalaldehyde (OPA). The procedure for CPZ included deproteinization with methanol, centrifugation and separation on a reversed phase column (C-18). Either of the drugs did not affect the determination of the other. The detection limits were 0.1 microgram/ml for ASTM and 0.5 microgram/ml for CPZ. These sensitivities seem good enough for a clinical application of the method considering usual dosage levels of the drugs.
氨基糖苷类抗生素与β-内酰胺类抗生素的联合疗法因疗效显著而在临床上经常使用。本文报道了一种用高效液相色谱法（HPLC）测定几种动物血液样本中天蓝霉素（ASTM）和头孢哌酮（CPZ）含量的方法。测定ASTM的步骤包括用CM-葡聚糖凝胶进行纯化、在反相柱（C-18）上分离以及用邻苯二甲醛（OPA）进行衍生化。测定CPZ的步骤包括用甲醇进行蛋白沉淀、离心以及在反相柱（C-18）上分离。两种药物中的任何一种都不会影响另一种药物的测定。ASTM的检测限为0.1微克/毫升，CPZ的检测限为0.5微克/毫升。考虑到药物的常用剂量水平，这些灵敏度对于该方法的临床应用来说似乎足够好了。
