Kobayashi S, Takai K, Inoue A
Jpn J Antibiot. 1986 Dec;39(12):3140-7.
Aminoglycoside and beta-lactam antibiotics are often used in combination. This paper reports a high performance liquid chromatographic determination method for each of astromicin (ASTM) and cefsulodin (CFS) in blood samples of several species. The procedure for ASTM included purification with CM-Sephadex, separation on a reversed phase column (C-18) and derivatization with o-phthalaldehyde (OPA). The procedure for CFS included deproteinization with methanol, centrifugation, filtration and separation on a reversed phase column (C-18). Either of the drugs did not affect the determination of the other. The detection limits were 0.1 microgram/ml for ASTM and 0.5 microgram/ml for CFS. These sensitivities seem good enough for a clinical application of the method considering usual dosage levels of the drugs.
氨基糖苷类抗生素和β-内酰胺类抗生素常联合使用。本文报道了几种动物血液样本中天蓝霉素（ASTM）和头孢磺啶（CFS）的高效液相色谱测定方法。ASTM的测定步骤包括用CM-葡聚糖凝胶纯化、在反相柱（C-18）上分离以及用邻苯二甲醛（OPA）衍生化。CFS的测定步骤包括用甲醇去蛋白、离心、过滤以及在反相柱（C-18）上分离。两种药物中的任何一种都不影响另一种的测定。ASTM的检测限为0.1微克/毫升，CFS的检测限为0.5微克/毫升。考虑到药物的常用剂量水平，这些灵敏度对于该方法的临床应用似乎足够好了。
