Laboratory for Marine Fisheries Science and Food Production Processes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, People's Republic of China.
School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, People's Republic of China.
Microb Ecol. 2023 Jul;86(1):112-126. doi: 10.1007/s00248-022-02036-2. Epub 2022 May 23.
Polychaetes are important benthic macrofauna that lives in sediments, usually in intertidal flats with high organic content and high sulfide. It has been suggested that polychaete bioturbation could perform environmental remediation. During the process, the microbial community plays important roles. Here, we used high-throughput sequencing technology to study the bioturbation effects on the bacterial community in the polychaete (Perinereis aibuhitensis) burrows at different tidal positions in intertidal flat. The results showed that the bacterial communities were dramatically influenced by the polychaete bioturbation. The ACE, Chao, and Shannon indices of the polychaete burrows increased in summer. Dominant phyla in the polychaete burrows were Proteobacteria, Campilobacterota, Desulfobacterota, Chloroflexi, and Bacteroidota, and the dominant bacterial families were Sulfurvaceae, Flavobacteriaceae, Rhodobacteraceae, Woeseiaceae, Desulfobulbaceae, and Sulfurimonadaceae. Results of linear discriminant analysis effect size (LEfSe) showed that groups that include organic matter degraders, such as Bacteroidota, Flavobacteriaceae, Rhodobacteraceae, Woeseiaceae, and groups that include sulfur oxidizers, such as Campilobacterota, Sulfurovaceae, Rhodobacteraceae, Desulfobulbaceae, and Sulfurimonadaceae, were significantly increased due to the polychaete bioturbation. The polychaete bioturbation reduced the complexity of the bacterial co-occurrence network while increased its modularity and homogeneity. The polychaete bioturbation also changed the functional groups, which significantly enhanced in functional groups of aerobic nitrite oxidation, nitration, dark thiosulfate oxidation, dark sulfur oxidation, and dark sulfite oxidation, while nitrogen respiration and nitrate respiration decreased. These results provide insight into the impact of bacterial communities under the intertidal polychaete bioturbation.
多毛类是生活在沉积物中的重要底栖大型动物，通常生活在富含有机物和高硫化物的潮间带。有人提出，多毛类生物扰动可以进行环境修复。在这个过程中，微生物群落起着重要的作用。在这里，我们使用高通量测序技术研究了不同潮位潮间带多毛类（沙蚕属 Perinereis aibuhitensis）洞穴中生物扰动对细菌群落的影响。结果表明，细菌群落受到多毛类生物扰动的显著影响。夏季，多毛类洞穴中的 ACE、Chao 和 Shannon 指数增加。多毛类洞穴中的优势门是变形菌门、Campilobacterota、脱硫杆菌门、绿弯菌门和拟杆菌门，优势细菌科是硫杆菌科、黄杆菌科、红杆菌科、Woeseiaceae、脱硫杆菌科和硫单胞菌科。线性判别分析效应量（LEfSe）的结果表明，由于多毛类生物扰动，包括有机物质降解者在内的群体，如拟杆菌门、黄杆菌科、红杆菌科、Woeseiaceae 以及包括硫氧化者在内的群体，如 Campilobacterota、硫杆菌科、红杆菌科、脱硫杆菌科和硫单胞菌科，显著增加。多毛类生物扰动降低了细菌共生网络的复杂性，同时增加了其模块性和同质性。多毛类生物扰动还改变了功能群，其中有氧亚硝酸盐氧化、硝化、暗硫代硫酸盐氧化、暗硫氧化和暗亚硫酸盐氧化的功能群显著增强，而氮呼吸和硝酸盐呼吸减少。这些结果为了解潮间带多毛类生物扰动下的细菌群落影响提供了线索。
