College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Biomolecules. 2023 Jan 30;13(2):254. doi: 10.3390/biom13020254.
To optimize the integrated multi-trophic aquaculture (IMTA) model, improve the efficiency of resource utilization, and reduce environmental pollution, as a potential bioremediation species, was studied to investigate the effect of culture on the dynamics and assembly of bacterial communities in sediment. Four experimental groups were established at four different densities: 0, 234.38, 468.75, and 937.5 g/m (represented as CON, LD, MD, and HD, respectively). Each group was with three replicates. The 16S ribosomal RNA (rRNA) high-throughput sequencing was used to evaluate the composition, function, and assembly of bacterial communities in sediment. cultivation significantly altered the composition and function of the bacterial communities in sediment; at high stocking density, it significantly decreased anaerobic and increased aerobic organic matter decomposition, whereas at low stocking density, it decreased the number of bacteria involved in sulfate reduction and inhibited the denitrification process. decreased direct competition and promoted collaboration or niche sharing in bacterial communities, especially at the high stocking density. Moreover, cultivation resulted in greater changes in the environmental factors. Variations in dissolved oxygen, pH, total nitrogen, nitrate, and nitrite levels were closely related to the altered composition and function of the bacterial communities. Stochastic processes dominated the bacterial community assembly in the sediment and cultivation had limited impacts on the bacterial community assembly. The study provided a reference for the dynamics and assembly of bacterial communities in sediment with different densities of cultivation and we hope that the findings will provide a theoretical reference for the optimization of IMTA and improve management strategies for polyculture.
为了优化综合多营养层水产养殖（IMTA）模式，提高资源利用效率，减少环境污染，作为一种有潜力的生物修复物种，研究了 对沉积物中细菌群落动态和组装的影响。在四个不同密度下建立了四个实验组：0、234.38、468.75 和 937.5 g/m（分别表示为 CON、LD、MD 和 HD）。每个组有三个重复。使用 16S 核糖体 RNA（rRNA）高通量测序来评估沉积物中细菌群落的组成、功能和组装。 养殖显著改变了沉积物中细菌群落的组成和功能；在高放养密度下，它显著降低了厌氧有机物质分解，增加了好氧有机物质分解，而在低放养密度下，它减少了参与硫酸盐还原的细菌数量，并抑制了反硝化过程。 养殖减少了细菌群落中的直接竞争，促进了合作或生态位共享，尤其是在高放养密度下。此外， 养殖导致环境因素发生更大变化。溶解氧、pH 值、总氮、硝酸盐和亚硝酸盐水平的变化与细菌群落组成和功能的改变密切相关。随机过程主导了沉积物中细菌群落的组装，而 养殖对细菌群落组装的影响有限。该研究为不同密度的 养殖沉积物中细菌群落的动态和组装提供了参考，我们希望这些发现将为 IMTA 的优化和改善 混养的管理策略提供理论参考。
