Lin Shau-Ching, Li Yi, Hu Fang-Yu, Wang Chih-Lu, Kuang Yun-Hung, Sung Chang-Lin, Tsai Shin-Fu, Yang Zhi-Wei, Li Charng-Pei, Huang Shou-Horng, Liao Chung-Ta, Hechanova Sherry Lou, Jena Kshirod K, Chuang Wen-Po
Department of Agronomy, National Taiwan University, Taipei, 10617, Taiwan.
Crop Improvement Division, Taoyuan District Agricultural Research and Extension Station, Council of Agriculture, 32745, Taoyuan City, Taiwan.
Bot Stud. 2022 May 23;63(1):16. doi: 10.1186/s40529-022-00347-8.
Nitrogen is an essential macronutrient for plant growth and development. Crops with a high nitrogen input usually have high yields. However, outbreaks of brown planthoppers (Nilaparvata lugens; BPH) frequently occur on rice farms with excessive nitrogen inputs. Rice plants carrying BPH resistance genes are used for integrated pest management. Thus, the impact of nitrogen on the resistance of rice near-isogenic lines (NILs) with BPH resistance genes was investigated.
We tested these NILs using a standard seedbox screening test and a modified bulk seedling test under different nitrogen treatments. The amount of nitrogen applied had an impact on the resistance of some lines with BPH resistance genes. In addition, three NILs (NIL-BPH9, NIL-BPH17, and NIL-BPH32) were further examined for antibiosis and antixenosis under varying nitrogen regimes. The N. lugens nymph population growth rate, honeydew excretion, female fecundity, and nymph survival rate on the three NILs were not affected by different nitrogen treatments except the nymph survival rate on NIL-BPH9 and the nymph population growth rate on NIL-BPH17. Furthermore, in the settlement preference test, the preference of N. lugens nymphs for IR24 over NIL-BPH9 or NIL-BPH17 increased under the high-nitrogen regime, whereas the preference of N. lugens nymphs for IR24 over NIL-BPH32 was not affected by the nitrogen treatments.
Our results indicated that the resistance of three tested NILs did not respond to different nitrogen regimes and that NIL-BPH17 exerted the most substantial inhibitory effect on N. lugens growth and development.
氮是植物生长发育必需的大量营养素。高氮投入的作物通常产量较高。然而，在氮投入过多的稻田中，褐飞虱（Nilaparvata lugens；BPH）暴发频繁。携带BPH抗性基因的水稻植株用于病虫害综合防治。因此，研究了氮对具有BPH抗性基因的水稻近等基因系（NILs）抗性的影响。
我们在不同氮处理下，使用标准育苗箱筛选试验和改良的大苗试验对这些NILs进行了测试。施氮量对一些具有BPH抗性基因的品系的抗性有影响。此外，对三个NILs（NIL-BPH9、NIL-BPH17和NIL-BPH32）在不同氮素水平下的抗生性和抗嗜性进行了进一步研究。除了NIL-BPH9上的若虫存活率和NIL-BPH17上的若虫种群增长率外，不同氮处理对这三个NILs上褐飞虱若虫种群增长率、蜜露排泄量、雌虫繁殖力和若虫存活率均无影响。此外，在栖居偏好试验中，在高氮条件下，褐飞虱若虫对IR24的偏好高于NIL-BPH9或NIL-BPH17，而褐飞虱若虫对IR24的偏好高于NIL-BPH32不受氮处理的影响。
我们的结果表明，三个测试的NILs的抗性对不同的氮素水平没有响应，并且NIL-BPH17对褐飞虱的生长发育具有最显著的抑制作用。
