Kamal Md Mostofa, Nguyen Cuong Dinh, Sanada-Morimura Sachiyo, Zheng Shao-Hui, Fujita Daisuke
The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.
Agrotechnology Discipline, Khulna University, Khulna, 9208, Bangladesh.
Breed Sci. 2023 Jun;73(3):278-289. doi: 10.1270/jsbbs.22093. Epub 2023 Jun 15.
The brown planthopper (BPH), Stål, is an insect pest that severely damages rice ( L.) in Asia, causing huge yield loss. Use of resistant variety is a cost-effective and eco-friendly strategy for maintaining BPH populations below the economic injury level. However, current BPH populations have been changed to virulence against resistant varieties. In this study, to estimate effective combinations among eight BPH resistance genes (, , , , , , and ), eight near-isogenic lines with the genetic background of an Indica Group rice variety 'IR64' (IR64-NIL) were developed using marker-assisted selection. The genome recoveries of these NILs ranged from 89.3% to 98.8% and agronomic traits of them were similar to those of 'IR64'. In modified seed box screening test, resistance level of IR64-NILs was higher than that of 'IR64'. In antibiosis test, high adult mortalities of BPH (from 56.0% to 97.0%) were observed among NILs, in comparison with that of 'IR64'. Among IR64-NILs, the line carrying showed the highest resistance level at all tests. Thus, these IR64-NILs with multiple BPH resistance genes could be valuable breeding lines for enhancing resistance levels by gene pyramiding and multiline variety.
褐飞虱（BPH），斯塔尔，是一种严重危害亚洲水稻（L.）的害虫，会造成巨大的产量损失。使用抗性品种是将褐飞虱种群数量维持在经济损害水平以下的一种经济有效且环保的策略。然而，目前的褐飞虱种群已对抗性品种产生了毒性变化。在本研究中，为了评估八个褐飞虱抗性基因（，，，，，，和）之间的有效组合，利用标记辅助选择培育了八个具有籼稻品种‘IR64’遗传背景的近等基因系（IR64-NIL）。这些近等基因系的基因组回收率在89.3%至98.8%之间，其农艺性状与‘IR64’相似。在改良的种子盒筛选试验中，IR64-NIL的抗性水平高于‘IR64’。在抗生性试验中，与‘IR64’相比，近等基因系中观察到褐飞虱的成虫死亡率较高（从56.0%到97.0%）。在IR64-NIL中，携带的品系在所有试验中表现出最高的抗性水平。因此，这些具有多个褐飞虱抗性基因的IR64-NIL可作为有价值的育种材料，通过基因聚合和多系品种来提高抗性水平。
