Department of Respiratory Medicine and Clinical Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan.
Department of Respiratory Medicine, National Hospital Organization Osaka Toneyama Medical Center, 5-1-1 Toneyama Toyonaka, Osaka, Japan.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005378.
A slowly growing mycobacteria, identified as strain TY59, was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the complex and closely related species. Strain TY59 has highest 16S rRNA gene sequence similarities to the type strains of (99.80 % sequence similarity), (99.74 %), (99.54 %), subsp. (99.54 %) and subsp. (99.54 %). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta () sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59 were and with 97.90-98.25 % identity in ITS and 96.4-96.6 % in . The strain's 65 kDa heat shock protein () gene was different from those of , and subsp. with 72.4-74.2 % identity. Average nucleotide identity results showed a 93.4 % match to as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 °C, negative results for nitrate reduction, β-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59 (RIMD 1371001=CIP 111917).
一株生长缓慢的分枝杆菌 TY59 株从一名患有肺炎的老年男性的痰液中分离得到。16S rRNA 基因序列分析表明，该菌株与复合群的成员相似，且与密切相关的种最为相似。TY59 株与 （99.80%序列相似性）、 （99.74%）、 （99.54%）、 亚属 （99.54%）和 subsp. （99.54%）的模式菌株的 16S rRNA 基因序列相似度最高。内部转录间隔区（ITS）和 DNA 指导的 RNA 聚合酶亚基β（）序列分析得出的结果与 16S rRNA 基因分析相似。与 TY59 株最为接近的种是 和 ，ITS 序列相似度为 97.90-98.25%， 序列相似度为 96.4-96.6%。该菌株的 65kDa 热休克蛋白（hsp65）基因与 、 和 subsp. 不同，其相似度分别为 72.4-74.2%。平均核苷酸同一性结果显示，与 相似度最高，为 93.4%。表型上，非产色、粗糙菌落、42°C 生长、硝酸盐还原、β-葡萄糖苷酶和吐温 80 水解阴性、过氧化氢酶活性阳性，使该菌株与密切相关的种相区别。我们建议将 sp. nov. 作为一种新的生长缓慢分枝杆菌种。模式菌株为 TY59（RIMD 1371001=CIP 111917）。
